Oxidoreductases use metallocofactors, organic molecules, and four amino acids (Tyr, Trp, Cys, and Gly) to perform electron transfer (ET) and proton-coupled electron transfer (PCET) reactions. These four amino acids serve as one-electron (radical) redox mediators in biocatalytic and multistep electron/hole transfer processes, some of which are essential to life on earth. There is also a sinister side to amino-acid ET/PCET since these reactions can be induced at oxidative stress conditions and cause significant cellular damage. It is extremely challenging to experimentally resolve the thermodynamic and kinetic redox properties of a single amino-acid residue. Consequently, key information to support the understanding of one of nature's essential redox tools is missing. Our research focuses on developing a family of well-structured model proteins with the unique capability of providing detailed mechanistic information on Tyr (Y) and Trp (W) oxidation-reduction reactions.
- Ph.D. in , Stockholm University - (Stockholm, Sweden) 1997
- B.S. in , Stockholm University - (Stockholm, Sweden) 1992
Academic Articles36
- Zhu, Q., Soudackov, A. V., Tommos, C., & Hammes-Schiffer, S. (2024). Proton-Coupled Electron Transfer upon Oxidation of Tyrosine in a De Novo Protein: Analysis of Proton Acceptor Candidates.. Biochemistry.
- Nilsen-Moe, A., Reinhardt, C. R., Huang, P., Agarwala, H., Lopes, R., Lasagna, M., ... Hammarstrm, L. (2024). Switching the proton-coupled electron transfer mechanism for non-canonical tyrosine residues in a de novo protein.. Chemical Science. 15(11), 3957-3970.
- Tommos, C. (2022). Insights into the Thermodynamics and Kinetics of Amino-Acid Radicals in Proteins.. Annual Review of Biophysics. 51(1), 453-471.
- Simeon, R. A., Zeng, Y. u., Chonira, V., Aguirre, A. M., Lasagna, M., Baloh, M., ... Chen, Z. (2021). Protease-stable DARPins as promising oral therapeutics.. Protein Engineering, Design and Selection. 34, gzab028.
- Reinhardt, C. R., Sequeira, R., Tommos, C., & Hammes-Schiffer, S. (2021). Computing Proton-Coupled Redox Potentials of Fluorotyrosines in a Protein Environment.. Journal of Physical Chemistry B. 125(1), 128-136.
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