Most bacterial viruses (phages) cause lysis of their host cell to release the progeny virions. Large phages elaborate an enzyme ("endolysin") to degrade the cell wall and also a small membrane protein ("holin"). The holin accumulates in the membrane and then, at a precisely scheduled time, suddenly forms a hole to allow release of endolysin through the cytoplasmic membrane to gain access to the wall. We use molecular genetics and biochemistry to study how this small protein is able to act as a molecular "clock" and punch holes in membranes. Small phages make single proteins which cause host lysis in a different way. This strategy is to target the host cell wall synthesis machinery; that is, the virus makes a "protein antibiotic" that causes lysis in the same way as antibiotics like penicillin by inhibiting an enzyme in the multi-step pathway of murein biosynthesis. Thus, when the infected cell tries to divide, it blows up, or lyses, because it can't make the new cell wall between the daughter cells. Remarkably, each of three different, small phages blocks a different step in the pathway. These small lysis proteins are models for a completely new class of antibacterial antibiotics. Also, the E. coli SlyD protein is required for this mode of lysis in one case. SlyD is a member of an ubiquitous family of proteins related to human "immunophilins," the targets of immune-suppression drugs. We study SlyD to learn about the role of this class of proteins in biology.
- McBee, D., Ravindran, A., Newkirk, H., Burrowes, B., Young, R. y., & Gonzalez, C. (2021). Complete Genome Sequence of Bradyrhizobium japonicum Podophage Paso.. Microbiology Resource Announcements. 10(5),
- Yu, Z., Yao, G., Vizoso-Pinto, M. G., Sun, L., Young, R. y., Gonzalez, C., & Liu, M. (2021). Complete Genome Sequence of Burkholderia gladioli Phage Maja.. Microbiology Resource Announcements. 10(5),
- Bartz, M., Yao, G., Le, T., Liu, M., Burrowes, B., Gonzalez, C., & Young, R. (2021). Complete Genome Sequence of Achromobacter xylosoxidans Myophage Mano.. Microbiology Resource Announcements. 10(2),
- Martin, C., Lessor, L., Clark, J., Le, T., Gill, J. J., Young, R., & Liu, M. (2021). Complete Genome Sequence of Klebsiella pneumoniae Myophage Muenster.. Microbiology Resource Announcements. 10(3),
- Manuel, N., Rushing, L., Ravindran, A., Newkirk, H., Burrowes, B., Young, R. y., & Gonzalez, C. (2021). Complete Genome Sequence of Rhizobium japonicum Podophage Pasto.. Microbiology Resource Announcements. 10(3),
- Stansfield, T., Troxler, M., Young, R., Russell, D., & Scott, J. (2016). Major Trauma Centres: are Vascular Surgeons missing a training opportunity?. British Journal of Surgery. 103, 25-25.
- Das, M., Bhowmick, T. S., Ahern, S. J., Young, R. F., & Gonzalez, C. (2013). Therapeutic and prophylactic application of phages to control Pierce's disease. PHYTOPATHOLOGY. 103(6), 34-34.
- Summer, E. J., Liu, M., Summer, N. S., Gill, J. J., Janes, C., & Young, R. (2011). Phage of sulfate reducing bacteria isolated from high saline environment. NACE - International Corrosion Conference Series.
- Duarte, I., Wang, G., Gill, J. J., Young, R. F., LiPuma, J. J., & Gonzalez, C. F. (2010). A broad host range tailocin from Burkholderia. PHYTOPATHOLOGY. 100(6), S31-S31.
- Duarte, I., Wang, G., Young, R. F., LiPuma, J., & Gonzalez, C. F. (2009). Characterization of a tailocin from Burkholderia. PHYTOPATHOLOGY. 99(6), S30-S30.
- Phage Lysis awarded by National Institute of General Medical Sciences - (Bethesda, Maryland, United States) 2020 - 2025
- Control of Virus Induced Lysis awarded by DHHS-NIH-National Institute of General Medical Science 2016 - 2020
- Implementing Galaxy for Community-based Phage Genomics awarded by National Science Foundation 2016 - 2020
- NIH Clinical Center and the Center for Phage Technology awarded by National Institutes of Health 2014 - 2016
- FFATA: Lipid Production and Extraction for Biodiesel awarded by National Science Foundation 2011 - 2015
- Kongari, Ramchander Rohit (2018-08). The Role of Spanins in Outer Membrane Disruption. (Doctoral Dissertation)
- Sperber, Anthony Michael (2017-12). Characterization of YisK, a Cell Shape Modifier and Enzyme in Bacillus subtilis. (Doctoral Dissertation)
- Cahill, Jesse L (2017-12). Molecular, Genetic, and Biochemical Analysis of Phage Lambda Lysis. (Doctoral Dissertation)
- Chamakura, Karthik Reddy (2017-05). Evolved to Break Free: Evolution and Diversity in Single Gene Lysis. (Doctoral Dissertation)
- Chen, Yi (2016-08). Regulation of Phage Lysis: Diverse Strategies and Targets. (Doctoral Dissertation)