I am testing the theory that the endoplasmic reticulum, ER is the circulatory network of the cell, connecting different organelles to each other, allowing them to share signals, lipids, and proteins.
I am particularly interested in how the cytoskeletal system of plants regulates the movement of the ER network. In interphase, the actinomyosin network drives movement of the ER, just as it drives the movement organelles through the cytoplasm in a process called cytoplasmic streaming, a phenomenon in plants, but not animal cells. Of the seventeen different myosin forms in plants, only six are involved in active cytoplasmic streaming. We are sorting out which of those six guide the different movements of the endoplasmic reticulum.
I am also interested in the nature of the nexus between the ER and other organelles, including the chloroplast, plasma membrane, and Golgi. I have recently shown that by photo-stimulating the nexus between the chloroplast and the ER, the directional flow within the ER can be reversibly altered. This ability to generate very localized ER stress may have application in a wide variety of fields - from finding cures for neurodegenerative diseases such as Alzheimer's syndrome to developing crops that can better-tolerate physiological heat stress and drought.
- Plant Biotechnology Institute - (Saskatoon, Saskatchewan, Canada), Postdoctoral Training 1986
- University of Saskatchewan - (Saskatoon, Saskatchewan, Canada), Postdoctoral Training 1984
- Oregon State University - (Corvallis, Oregon, United States), Postdoctoral Training 1982
- Ph.D. in Biology, Stanford University - (Stanford, California, United States) 1981
- B.S. in Biology, University of Utah - (Salt Lake City, Utah, United States) 1976
- Griffing, L. R., Lin, C., Perico, C., White, R. R., & Sparkes, I. (2017). Plant ER geometry and dynamics: biophysical and cytoskeletal control during growth and biotic response. PROTOPLASMA. 254(1), 43-56.
- Cheng, X., Lang, I., Adeniji, O. S., & Griffing, L. (2017). Plasmolysis-deplasmolysis causes changes in endoplasmic reticulum form, movement, flow, and cytoskeletal association.. JOURNAL OF EXPERIMENTAL BOTANY. 68(15), 4075-4087.
- Griffing, L. R., Gao, H. T., & Sparkes, I. (2014). ER network dynamics are differentially controlled by myosins XI-K, XI-C, XI-E, XI-I, XI-1, and XI-2 e. Frontiers in Plant Science. 5(MAY), 218.
- Griffing, L. R. (2011). Laser Stimulation of the Chloroplast/Endoplasmic Reticulum Nexus in Tobacco Transiently Produces Protein Aggregates (Boluses) within the Endoplasmic Reticulum and Stimulates Local ER Remodeling. Molecular Plant. 4(5), 886-895.
- Griffing, L. R. (2011). WHO INVENTED THE DICHOTOMOUS KEY? RICHARD WALLER'S WATERCOLORS OF THE HERBS OF BRITAIN. American Journal of Botany. 98(12), 1911-1923.
- Griffing, L. R. (2018). Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and Its Partners and of Movement Within Its Tubules.. Methods in molecular biology (Clifton, N.J.). Methods in Molecular Biology. (pp. 75-102). Springer New York.
- Fowke, L. C., Griffing, L. R., Mersey, B. G., & Tanchak, M. A. (2018). Protoplasts for studies of cell organelles. Plant Protoplasts. (pp. 39-52). CRC Press.
- Griffing, L. R. (2010). Networking in the endoplasmic reticulum. Biochem Soc Trans. 38(3), 747-753.
- Griffing, L. R. (2008). FRET analysis of transmembrane flipping of FM4-64 in plant cells: is FM4-64 a robust marker for endocytosis?. JOURNAL OF MICROSCOPY. 231(2), 291-298.
- Bernhardt, S. P., & Griffing, L. R. (2001). An evaluation of image analysis at benthic sites based on color segmentation. BULLETIN OF MARINE SCIENCE. 69(2), 639-653.
- Kennedy, C. J., & Griffing, L. R. (2001). Quantitative analysis of transnuclear and intranuclear membranes in KDEL-GFP-expressing tobacco suspension culture cells. MOLECULAR BIOLOGY OF THE CELL. 12, 232A-232A.
- Enloe, L. C., & Griffing, L. R. (2000). Improved volume rendering for the visualization of living cells examined with confocal microscopy. MATHEMATICAL MODELING AND ESTIMATION TECHNIQUES IN COMPUTER VISION. 3960, 385-392.