overview

My lab uses X-ray crystallography to better understand the relationship between proteins and ligands. Tiny differences in the structure of a molecule can radically change the interaction between a protein and ligand and we are only begining to understand how many factors play a role in this interaction. By manipulating the individual components of a compound it is possible to create a chemical that binds to the protein better than the natural substrate, and prevent the natural reaction from occurring. This is the basis for rational drug design. Our efforts have lead us to collaborations with other labs and scientists in many disciplines as our approach to directed compound design has applications not only in basic research but also in pesticide development, health research and clinical research.

selected publications
Academic Articles352
  • Wang, H., Conover, G. M., Han, S., Sacchettini, J. C., & Han, A (2021). Development of single-cell-level microfluidic technology for long-term growth visualization of living cultures of Mycobacterium smegmatis. Microsystems & Nanoengineering. 7(1), 37.
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  • Mellott, D. M., Torres, D., Krieger, I. V., Cameron, S. A., Moghadamchargari, Z., Laganowsky, A., ... Harris, L. D (2021). Mechanism-Based Inactivation of Mycobacterium tuberculosis Isocitrate Lyase 1 by (2R,3S)-2-Hydroxy-3-(nitromethyl)succinic acid. Journal of the American Chemical Society. 143(42), 17666-17676.
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  • Black, K. A., Duan, L., Mandyoli, L., Selbach, B. P., Xu, W., Ehrt, S., Sacchettini, J. C., & Rhee, K. Y. (2021). Metabolic bifunctionality of Rv0812 couples folate and peptidoglycan biosynthesis in Mycobacterium tuberculosis. 218(7), e20191957.
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  • Pandey, S., Singh, A., Yang, G., d’Andrea, F. B., Jiang, X., Hartman, T. E., ... Burns-Huang, K. (2021). Characterization of Phosphopantetheinyl Hydrolase from Mycobacterium tuberculosis. 9(2), e00928-e00921.
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  • Pham, T. V., Mellott, D. M., Moghadamchargari, Z., Chen, K., Krieger, I., Laganowsky, A., Sacchettini, J. C., & Meek, T. D. (2021). Covalent Inactivation of Mycobacterium tuberculosis Isocitrate Lyase by cis-2,3-Epoxy-Succinic Acid. 16(3), 463-470.
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Chapters5
  • Lücke, C., Fushman, D., Ludwig, C., Hamilton, J. A., Sacchettini, J. C., & Rüterjans, H. (1999). A comparative study of the backbone dynamics of two closely related lipid binding proteins: Bovine heart fatty acid binding protein and porcine ileal lipid binding protein. Lipid Binding Proteins within Molecular and Cellular Biochemistry. 109-121. Springer US.
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  • Scapin, G., Young, A., Kromminga, A., Veerkamp, J. H., Gordon, J. I., & Sacchettini, J. C. (1993). High resolution X-ray studies of mammalian intestinal and muscle fatty acid-binding proteins provide an opportunity for defining the chemical nature of fatty acid: protein interactions. Cellular Fatty Acid-Binding Proteins II. 3-13. Springer US.
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  • Cistola, D. P., Sacchettini, J. C., & Gordon, J. I. (1990). 13C NMR studies of fatty acid-protein interactions: comparison of homologous fatty acid-binding proteins produced in the intestinal epithelium. Cellular Fatty Acid-binding Proteins. 101-110. Springer US.
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  • Musa, T. L., Ioerger, T. R., & Sacchettini, J. C THE TUBERCULOSIS STRUCTURAL GENOMICS CONSORTIUM: A STRUCTURAL GENOMICS APPROACH TO DRUG DISCOVERY. Advances in protein chemistry and structural biology. STRUCTURAL GENOMICS, PART C. 41-76.
Conference Papers48
  • Kieser, K. J., Baranowski, C., Chao, M. C., Long, J. E., Sassetti, C. M., Waldor, M. K., ... Rubin, E. J (2015). Peptidoglycan synthesis in Mycobacterium tuberculosis is organized into networks with varying drug susceptibility. Proceedings of the National Academy of Sciences of the United States of America. 112(42), 13087-13092.
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  • Xie, H., Rao, J., Mire, J., Sacchettini, J. C., Kong, Y., & Cirillo, J. D. (2010). Mtb Bla-specific fluorescent probes for tuberculosis detection and imaging. 240,
  • Mosser, R., Reddy, M., Bruning, J., Sacchettini, J. C., & Reinhart, G. D (2010). Redefining the Role of the Quaternary Shift in the Allosteric Inhibition of Bacillus Stearothermophilus Phosphofructokinase. Biophysical Journal. 98(3), 39a-39a.
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  • Pai, R., Sacchettini, J. C., & Ioerger, T. R (2008). Analysis of protein-ligand interactions using localized stereochemical features. Proceedings of the 2008 International Conference on Bioinformatics and Computational Biology, BIOCOMP 2008. 666-673.
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in the news
patents
Patents13
  • Reddy, M., Sacchettini, J. C., Zhou, N. E., & McClutchen, B. F. (2020). Compositions and methods for drug sensitization of parasites.
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  • Reddy, M., Sacchettini, J. C., Zhou, N. E., & Mccutchen, B. F. (2019). Compositions and methods for inhibition of mycobacteria.
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  • Sacchettini, J. C., Miller, M. W., Wallis, D., Zhou, N. E., & Fossum, T. W. (2019). Compositions and methods for drug sensitization of parasites.
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  • Sacchettini, J. C., Miller, M. W., Wallis, D., Zhou, N. E., & Fossum, T. W. (2019). Compositions and methods for drug sensitization of parasites.
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  • Reddy, M., Sacchettini, J. C., Zhou, N. E., & McCutchen, B. F. (2019). Compositions and methods for inhibition of mycobacteria.
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chaired theses and dissertations
Email
sacchett@tamu.edu
First Name
James
Last Name
Sacchettini
mailing address
Texas A&M University; Biochemistry & Biophysics; 2128 TAMU
College Station, TX 77843-2128
USA