Zhang, Junjie individual record
Associate Professor
overview

The living cell contains a collection of molecular machines to grow and function. These machines include the ribosomes, the chaperons, the proteasomes and other enzymes. Malfunction of these machines, if occurred in human, are related to many diseases. Understanding their three-dimensional (3D) structures is essential to understand how these machines work in the cell and eventually to treat those related diseases.



Here we use an experimental technique called cryo-electron microscopy (cryo-EM) to image these cellular machines in their native environment at liquid nitrogen temperatures. We then use image processing and graphics techniques to visualize their 3D structures, answering the questions such as how they assemble and how they interact with each other.



In addition, we develop computational modeling tools to interpret and animate these obtained 3D structures to further describe their movements and dynamics.

selected publications
Academic Articles35
  • Cui, Z., Li, X., Shin, J., Gamper, H., Hou, Y., Sacchettini, J. C., & Zhang, J. (2022). Interplay between an ATP-binding cassette F protein and the ribosome from Mycobacterium tuberculosis. Nature Communications. 13(1), 432.
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  • Jiang, M., Shin, J., Simeon, R., Chang, J., Meng, R., Wang, Y., ... Zhang, J. (2022). Structural dynamics of receptor recognition and pH-induced dissociation of full-length Clostridioides difficile Toxin B. PLOS Biology. 20(3), e3001589-e3001589.
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  • Chang, J., Gorzelnik, K. V., Thongchol, J., & Zhang, J. (2022). Structural Assembly of Q Virion and Its Diverse Forms of Virus-like Particles. Viruses. 14(2), 225-225.
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  • Gorzelnik, K. V., & Zhang, J. (2021). Cryo-EM reveals infection steps of single-stranded RNA bacteriophages. Progress in Biophysics and Molecular Biology. 160, 79-86.
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  • Shen, L., Su, Z., Yang, K., Wu, C., Becker, T., Bell-Pedersen, D., Zhang, J., & Sachs, M. S. (2021). Structure of the translating Neurospora ribosome arrested by cycloheximide. Proceedings of the National Academy of Sciences. (48), e2111862118.
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Chapters1
  • Zhang, J., Gorzelnik, K. V., Schmid, M. F., & Chiu, W. (2016). Single-Particle CryoEM of Macromolecular Complexes. Encyclopedia of Cell Biology. 5-13. Elsevier.
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Conference Papers1
  • Pintilie, G., Zhang, J., Chiu, W., & Gossard, D. (2009). Identifying Components in 3D Density Maps of Protein Nanomachines by Multi-scale Segmentation.. IEEE NIH Life Sci Syst Appl Workshop, 2009 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA) Formerly known as LSSA and. 2009, 44-47.
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Repository Documents / Preprints1
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Email
junjiez@tamu.edu
First Name
Junjie
Last Name
Zhang
mailing address
Texas A&M University; Biochemistry & Biophysics; 3474 TAMU
College Station, TX 77843-3474
USA