My laboratory studies the regulation of microvascular function at the level of arterioles in the retinal and coronary circulations. Sufficient blood flow supply of oxygen and nutrients to tissues to maintain normal function is controlled in large part by changes in the diameter of arterioles. Vasoconstriction or vasodilation of these small arteries will decrease or increase blood flow and nutrient delivery to the tissue, respectively. Two key chemical factors that are produced within the endothelial cells of blood vessels to control their diameter are nitric oxide (NO), a vasodilator, and endothelin-1, a vasoconstrictor. An imbalance in the production and/or release of these vasoactive factors has been implicated in the early stages of several cardiovascular diseases, but the underlying mechanisms contributing to these pathophysiological changes remain unclear. To address this knowledge gap, our research focuses on identifying cellular and molecular mechanisms that contribute to the vasomotor responses of arterioles to NO and endothelin-1 under conditions of health and disease. Current approaches that we use to investigate these mechanisms in the microcirculation include isolated and perfused arterioles, cultured vascular endothelial and smooth muscle cells, biochemical and molecular techniques (for detection of NO, superoxide anion, protein, and mRNA in arterioles), pharmacological and silencing RNA (siRNA) treatments, and blood flow velocity assessment via Doppler ultrasound.
- Texas A&M University - (College Station, Texas, United States), Postdoctoral Training 1999
- Ph.D. in Medical Sciences, Texas A&M University - (College Station, Texas, United States) 1997
- B.A. in Biology, St. Olaf College - (Northfield, Minnesota, United States) 1992
- Frederick, N. E., Mitchell, R., Hein, T. W., & Bagher, P. (2019). Morphological and pharmacological characterization of the porcine popliteal artery: A novel model for study of lower limb arterial disease. Microcirculation (New York, N.Y. : 1994). 26(6), e12527-e12527.
- Chen, Y., Xu, W., Rosa, R. H., Kuo, L., & Hein, T. W. (2019). Hyperglycemia Enhances Constriction of Retinal Venules via Activation of the Reverse-Mode Sodium-Calcium Exchanger. DIABETES. 68(8), 1624-1634.
- Mathur, T., Singh, K. A., Pandian, N., Tsai, S., Hein, T. W., Gaharwar, A. K., Flanagan, J. M., & Jain, A. (2019). Organ-on-chips made of blood: endothelial progenitor cells from blood reconstitute vascular thromboinflammation in vessel-chips. Lab on a Chip - Miniaturisation for Chemistry and Biology. 19(15), 2500-2511.
- Hein, T. W., Xu, X., Ren, Y., Xu, W., Tsai, S. H., Thengchaisri, N., & Kuo, L. (2019). Requisite roles of LOX-1, JNK, and arginase in diabetes-induced endothelial vasodilator dysfunction of porcine coronary arterioles. J Mol Cell Cardiol. 131, 82-90.
- Tsai, S. H., Xie, W., Zhao, M., Rosa, R. H., Hein, T. W., & Kuo, L. (2018). Alterations of Ocular Hemodynamics Impair Ophthalmic Vascular and Neuroretinal Function. American Journal Of Pathology. 188(3), 818-827.
- Kuo, L., Thengchaisri, N., & Hein, T. W. (2011). Regulation of Coronary Vasomotor Function by Oxidative Stress. Kimchi, A. (Eds.), New Frontiers in Heart Disease, Proceedings of the 16th World Congress of Heart Disease, International Academy of Cardiology Annual Scientific Sessions. (pp. 103-107). Medimond International Proceedings.
- Kuo, L., & Hein, T. W. (2003). Mechanism of shear stress-induced coronary microvascular dilation. Barth, F. G., Humphrey, J., & Secomb, T. W. (Eds.), Sensors and Sensing in Biology and Engineering. (pp. 197-212). Springer Vienna.
- Xie, W., Zhao, M., Tsai, S., Burkes, W., Ren, Y. i., Xu, W., ... Rosa, R. H. (2016). Correlation of spectral domain optical coherence tomography and histology in the porcine retina. Investigative Ophthalmology & Visual Science. 57(12),