overview

Dr. Kapler's broad research interests are concerned with the replication and transmission of eukaryotic chromosomes. The failure to completely replicate the genome during S phase or partially re-replicate chromosomes leads to genome instability- a hallmark of cancer cells. The central questions investigated in the laboratory are concerned with how replication initiation sites are established in chromosomes and how they are regulated during conventional (G1/S/G2/M) and alternative cell cycles, including endoreplication (gap-S-gap-S...) and locus-specific gene amplification. The current focus of the lab is to use high throughput (nascent strand) DNA sequencing to generate a comprehensive map of replication initiation sites under different physiological conditions.

selected publications
Academic Articles25
  • Feng, L., Wang, G., Hamilton, E. P., Xiong, J., Yan, G., Chen, K., ... Liu, Y (2017). A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.. Nucleic Acids Res. 45(16), 9481-9502.
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  • Donti, T. R., Datta, S., Sandoval, P. Y., & Kapler, G. M (2009). Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins.. EMBO J. 28(3), 223-233.
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  • Mohammad, M. M., Donti, T. R., Sebastian Yakisich, J., Smith, A. G., & Kapler, G. M (2007). Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition.. EMBO J. 26(24), 5048-5060.
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  • Morrison, T. L., Yakisich, J. S., Cassidy-Hanley, D., & Kapler, G. M (2005). TIF1 Represses rDNA replication initiation, but promotes normal S phase progression and chromosome transmission in Tetrahymena.. Mol Biol Cell. 16(6), 2624-2635.
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Chapters1
  • Gaertig, J., & Kapler, G (2000). Transient and stable DNA transformation of Tetrahymena thermophila by electroporation.. METHODS IN CELL BIOLOGY, VOL 49. Methods in Cell Biology. 485-500. Elsevier.
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Conference Papers1
  • KAPLER, G. M., & BLACKBURN, E. H (1994). A WEAK EXCISION MUTATION BLOCKS DEVELOPMENTALLY-CONTROLLED AMPLIFICATION OF THE TETRAHYMENA-THERMOPHILA RDNA MINICHROMOSOME. JOURNAL OF CELLULAR BIOCHEMISTRY. 100-100.
chaired theses and dissertations
Email
gkapler@tamhsc.edu
First Name
Geoffrey
Last Name
Kapler
mailing address
Texas A&M Health Science Center; Instructional Admin; 1114 TAMUS
College Station, TX 77843-1114
USA