Dr. Kapler's broad research interests are concerned with the replication and transmission of eukaryotic chromosomes. The failure to completely replicate the genome during S phase or partially re-replicate chromosomes leads to genome instability- a hallmark of cancer cells. The central questions investigated in the laboratory are concerned with how replication initiation sites are established in chromosomes and how they are regulated during conventional (G1/S/G2/M) and alternative cell cycles, including endoreplication (gap-S-gap-S...) and locus-specific gene amplification. The current focus of the lab is to use high throughput (nascent strand) DNA sequencing to generate a comprehensive map of replication initiation sites under different physiological conditions.
- University of California, San Francisco - (San Francisco, California, United States), Postdoctoral Training 1994
- Ph.D. in Genetics, Harvard University - (Cambridge, Massachusetts, United States) 1989
- B.S. in Biology, University of Connecticut - (Storrs, Connecticut, United States) 1979
Academic Articles32
- Lee, S. R., Pollard, D. A., Galati, D. F., Kelly, M. L., Miller, B., Mong, C., ... Turman, G. (2021). Disruption of a 23-24 nucleotide small RNA pathway elevates DNA damage responses in Tetrahymena thermophila.. Molecular Biology of the Cell. 32(15), 1335-1346.
- Liu, Y., Nan, B., Niu, J., Kapler, G. M., & Gao, S. (2021). An Optimized and Versatile Counter-Flow Centrifugal Elutriation Workflow to Obtain Synchronized Eukaryotic Cells.. Frontiers in Cell and Developmental Biology. 9, 664418.
- Zhao, Q., Zhang, L., Hai, B. o., Wang, J., Baetge, C. L., Deveau, M. A., ... Liu, F. (2020). Transient Activation of the Hedgehog-Gli Pathway Rescues Radiotherapy-Induced Dry Mouth via Recovering Salivary Gland Resident Macrophages.. Cancer Research. 80(24), 5531-5542.
- Feng, L., Wang, G., Hamilton, E. P., Xiong, J., Yan, G., Chen, K., ... Liu, Y. (2017). A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.. Nucleic Acids Research (NAR). 45(16), 9481-9502.
- Sandoval, P. Y., Lee, P., Meng, X., & Kapler, G. M. (2015). Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena.. PLoS Genetics. 11(7), e1005405-e1005405.
Chapters1
- Gaertig, J., & Kapler, G. (2000). Transient and stable DNA transformation of Tetrahymena thermophila by electroporation.. Methods in Cell Biology. Methods in Cell Biology Volume 62. 485-500. Elsevier.
Conference Papers1
- KAPLER, G. M., & BLACKBURN, E. H. (1994). A WEAK EXCISION MUTATION BLOCKS DEVELOPMENTALLY-CONTROLLED AMPLIFICATION OF THE TETRAHYMENA-THERMOPHILA RDNA MINICHROMOSOME. Journal of Cellular Biochemistry. 100-100.
Principal Investigator1
- BICH285 Directed Studies Instructor
- BICH491 Hnr-research Instructor
- BICH491 Research Instructor
- BICH491 Research:in-ab Instructor
- BICH625 Nuc Acid-prot Interact Instructor
- Meng, Xiangzhou (2015-05). Distinct DNA Replication Programs in Tetrahymena Thermophila. (Doctoral Dissertation)
