Kapler, Geoffrey
individual record
Professor and Chair
Positions:
- Professor, Biochemistry and Biophysics, College of Agriculture and Life Sciences
- Professor and Chair, Cell Biology and Genetics, School of Medicine
overview
Dr. Kapler's broad research interests are concerned with the replication and transmission of eukaryotic chromosomes. The failure to completely replicate the genome during S phase or partially re-replicate chromosomes leads to genome instability- a hallmark of cancer cells. The central questions investigated in the laboratory are concerned with how replication initiation sites are established in chromosomes and how they are regulated during conventional (G1/S/G2/M) and alternative cell cycles, including endoreplication (gap-S-gap-S...) and locus-specific gene amplification. The current focus of the lab is to use high throughput (nascent strand) DNA sequencing to generate a comprehensive map of replication initiation sites under different physiological conditions.
education and training
- University of California, San Francisco - (San Francisco, California, United States), Postdoctoral Training 1994
- Ph.D. in Genetics, Harvard University - (Cambridge, Massachusetts, United States) 1989
- B.S. in Biology, University of Connecticut - (Storrs, Connecticut, United States) 1979
selected publications
Academic Articles32
- Lee, S. R., Pollard, D. A., Galati, D. F., Kelly, M. L., Miller, B., Mong, C., ... Turman, G. (2021). Disruption of a 23-24 nucleotide small RNA pathway elevates DNA damage responses in Tetrahymena thermophila.. Mol Biol Cell. 32(15), 1335-1346.
- Liu, Y., Nan, B., Niu, J., Kapler, G. M., & Gao, S. (2021). An Optimized and Versatile Counter-Flow Centrifugal Elutriation Workflow to Obtain Synchronized Eukaryotic Cells.. Front Cell Dev Biol. 9, 664418.
- Zhao, Q., Zhang, L., Hai, B. o., Wang, J., Baetge, C. L., Deveau, M. A., ... Liu, F. (2020). Transient Activation of the Hedgehog-Gli Pathway Rescues Radiotherapy-Induced Dry Mouth via Recovering Salivary Gland Resident Macrophages.. Cancer Res. 80(24), 5531-5542.
- Feng, L., Wang, G., Hamilton, E. P., Xiong, J., Yan, G., Chen, K., ... Liu, Y. (2017). A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.. Nucleic Acids Res. 45(16), 9481-9502.
- Sandoval, P. Y., Lee, P., Meng, X., & Kapler, G. M. (2015). Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena.. PLoS Genet. 11(7), e1005405-e1005405.
Chapters1
- Gaertig, J., & Kapler, G. (2000). Transient and stable DNA transformation of Tetrahymena thermophila by electroporation.. Methods in Cell Biology Volume 62. 485-500. Elsevier.
Conference Papers1
- KAPLER, G. M., & BLACKBURN, E. H. (1994). A WEAK EXCISION MUTATION BLOCKS DEVELOPMENTALLY-CONTROLLED AMPLIFICATION OF THE TETRAHYMENA-THERMOPHILA RDNA MINICHROMOSOME. JOURNAL OF CELLULAR BIOCHEMISTRY. 100-100.
researcher on
Principal Investigator1
awards and honors
recent teaching activities
- BICH285 Directed Studies Instructor
- BICH491 Hnr-research Instructor
- BICH491 Research Instructor
- BICH625 Nuc Acid-prot Interact Instructor
- BICH691 Research Instructor
chaired theses and dissertations
- Meng, Xiangzhou (2015-05). Distinct DNA Replication Programs in Tetrahymena Thermophila. (Doctoral Dissertation)
- Lee, Po-Hsuen (2014-05). Regulation of DNA Replication During Conventional and Unconventional Cell Cycles in Tetrahymena. (Doctoral Dissertation)
- Sandoval Oporto, Pamela (2011-05). Conserved and Unconventional Responses to DNA Damage in Tetrahymena. (Doctoral Dissertation)
- Datta, Shibani (2005-12). Isolation and genetic dissection of an eukaryotic replicon that supports autonomous DNA replication. (Master's Thesis)
- Morrison, Tara Laine (2005-08). Roles of the Tetrahymena thermophila type I element binding factor, TIF1, in DNA replication and genome stability. (Doctoral Dissertation)
Email
gkapler@tamhsc.edu
First Name
Geoffrey
Last Name
Kapler
mailing address
Texas A&M Health Science Center; Instructional Admin; 1114 TAMUS
College Station, TX 77843-1114
USA