My research focuses on proteomics, lipidomics, biophysical chemistry and application and development of mass spectrometry, such as "label-free" nano-particle based biosensors and novel peptide/protein isolation and purification strategies. We are also investigating the structure(s) of model peptides in an effort to better describe folding/unfolding and structure of membrane and intrinsically disordered (IDP) proteins. Peptides take on very different 2?, 3? and 4? structure, which determine or influence bio-activity. In the presence of lipid vesicles peptides can exist as solution-phase species, "absorbed" on lipid bilayers or "inserted" (as a monomer or multimer) in lipid bilayers. By what mechanism do peptides interact with lipid membranes to affect these structural changes, how do peptide-lipid interactions promote self-assembly to form intermediates that eventually yield aggregates, i.e., amyloid fibrils, or how does metal ion coordination affect the structure of metalloproteins? Mass spectrometry-based experiments, hydrogen/deuterium (H/D) exchange, chemical 'foot-printing' and gas-phase (ion-molecule and ion-ion reaction chemistry) and solution-phase chemical modifications, have expanded our abilities to address such questions, and new instrumental approaches, esp. ion mobility spectrometry (IMS) combined with enhanced molecular dynamics simulations (MDS), have become standard tools for structural-mass spectrometry studies. Over the past several years we have either acquired or developed novel, next-generation IM-MS instruments that are redefining cutting-edge structural-mass spectrometry research as well as cutting-edge computational tools essential to carry out these studies. Our new laboratories in the Interdisciplinary Life Sciences Building (ILSB) provides exciting opportunities for collaborative, interdisciplinary research with chemical-biologists, biochemists and other chemists.
Academic Articles342
- McCabe, J. W., Jones, B. J., Walker, T. E., Schrader, R. L., Huntley, A. P., Lyu, J., ... Russell, D. H. (2021). Implementing Digital-Waveform Technology for Extended m/z Range Operation on a Native Dual-Quadrupole FT-IM-Orbitrap Mass Spectrometer. Journal of the American Society for Mass Spectrometry. 32(12), 2812-2820.
- Loo, J. A., Russell, D. H., & Wang, Y. (2021). Editorial: Focus on Protein Footprinting, Honoring Michael Gross, Recipient of the 2020 John B. Fenn Award for a Distinguished Contribution in Mass Spectrometry.. J Am Soc Mass Spectrom. 32(7), 1565-1566.
- Raab, S. A., El-Baba, T. J., Laganowsky, A., Russell, D. H., Valentine, S. J., & Clemmer, D. E. (2021). Protons Are Fast and Smart; Proteins Are Slow and Dumb: On the Relationship of Electrospray Ionization Charge States and Conformations.. J Am Soc Mass Spectrom. 32(7), 1553-1561.
- El-Baba, T. J., Raab, S. A., Buckley, R. P., Brown, C. J., Lutomski, C. A., Henderson, L. W., ... Clemmer, D. E. (2021). Thermal Analysis of a Mixture of Ribosomal Proteins by vT-ESI-MS: Toward a Parallel Approach for Characterizing the Stabilitome.. Anal Chem. 93(24), 8484-8492.
- Wang, Y., & Russell, D. (2021). A SPECIAL ISSUE DEDICATED TO THE OUTSTANDING SCIENTIFIC CAREER OF PROF. MICHAEL L. GROSS.. Mass Spectrom Rev. 40(3), 161-161.
Chapters7
- May, J., & Russell, D. (2010). A Cryogenic-Temperature Ion Mobility Mass Spectrometer for Improved Ion Mobility Resolution. Ion Mobility Spectrometry - Mass Spectrometry. 137-151. CRC Press.
- Russell, D. H., Oriedo, J., & Solouki, T. (1996). Development of a Fourier-Transform Ion Cyclotron Resonance (FTICR) Mass Spectrometry Method for Studies of Metal Ion Excited States. Organometallic Ion Chemistry. 197-228. Springer Netherlands.
- Wang, Y., Yurttas, L., Dale, B. E., Russell, D. H., Kinsel, G. R., Preston, L. M., Wright, M. S., & Hayes, T. K. (1994). Matrix-Assisted Laser Desorption Mass Spectrometry To Monitor Synthesis And Folding of Manduca Sexta Eclosion Hormone And Its Analogs. Peptides. 265-267. Springer Netherlands.
- Russell, D. H., Fredeen, D. A., & Tecklenburg, R. E. (1989). Structure—Reactivity Relationships for Ionic Transition Metal Carbonyl Cluster Fragments. Gas Phase Inorganic Chemistry. 115-135. Springer US.
- Russell, D. H., & Castro, M. E. (1986). Desorption Ionization and Fourier Transform Mass Spectrometry for the Analysis of Large Biomolecules. X-RAY LASERS 2016. Springer Proceedings in Physics. 209-212. Springer Berlin Heidelberg.
Conference Papers77
- Liu, Y., Liu, W., Poltash, M., Russell, D., & Laganowsky, A. (2019). Native ion mobility mass spectrometry of membrane protein complexes. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 257,
- Russell, D. (2018). Native-ESI and ion mobility-mass spectrometry: Expanding MS-based structural biology through understanding of chemical fundamentals. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 255,
- Russell, D. (2017). Mapping peptide/protein conformation space: A challenge for IM-MS. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 253,
- Rogers, D., El-Baba, T., Shi, L., Khan, F., Kim, D., Hales, D., Russell, D., & Clemmer, D. (2016). Histidine-modified polyproline-13 explores the nature of two-state cooperativity. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 251,
- Russell, D. (2016). Mass spectrometry and structural biology: Ion mobility-mass spectrometry studies of water and water-mediated conformational preferences of peptides and proteins. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 251,
Repository Documents / Preprints3
- Qiao, P., Schrecke, S., Walker, T., McCabe, J., Lyu, J., Zhu, Y., ... Laganowsky, A. (2021). Entropy in the molecular recognition of membrane protein-lipid interactions.
- Walker, T. E., Shirzadeh, M., Sun, H. M., McCabe, J. W., Roth, A., Moghadamchargari, Z., ... Russell, D. H. (2021). Temperature Regulates Stability, Ligand Binding (Mg2+ and ATP) and Stoichiometry of GroEL/GroES Complexes.
- Patra, S., Lin, C., Ghosh, M. K., Havens, S. M., Cory, S. A., Russell, D. H., & Barondeau, D. P. (2020). Recapitulating the frataxin activation mechanism in an engineered bacterial cysteine desulfurase supports the architectural switch model.
Patents5
- Mclean, J., Russell, D., Egan, T., Ugarov, M., & Schultz, A. (2010). Multiplex data acquisition modes for ion mobility-mass spectrometry.
- Mclean, J., Russell, D., Egan, T., Ugarov, M., & Schultz J. A. (2008). Multiplex data acquisition modes for ion mobility-mass spectrometry.
- Russell, D., & Mclean, J. (2007). Advanced optics for rapidly patterned laser profiles in analytical spectrometry.
- Mclean, J., Russell, D., & Schultz, A. (2006). Gas-phase purification of biomolecules by ion mobility for patterning microarrays and protein crystal growth.
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Principal Investigator3
- CHEM315 Fund. Of Quant. Analysis Instructor
- CHEM415 Analytical Chemistry Instructor
- CHEM491 Hnr-research Instructor
- CHEM491 Research Instructor
- CHEM491 Research: In-ab Instructor
- Kim, Doyong (2016-08). Monitorying the Desolvaiton of Ions and Create Candidate Structure for the Ions Detected by Ion Mobility-Mass Spectrometry Study by Molecular Dynamics Simulations. (Doctoral Dissertation)
- Servage, Kelly Anne (2016-08). Investigating the Effects of Dehydration on the Conformational Preferences of Biomolecules during the Final Stages of Electrospray Ionization. (Doctoral Dissertation)
- Lang, Phillip (2016-05). An Investigation of Polymer Encapsualted Gold Nanoparticles for Use in Analytical Methods for Proteomic Based Research. (Master's Thesis)
- Xiao, Chunying (2015-12). Effects of Charge States, Charge Sites and Side Chain Interactions on Conformational Preferences of a Series of Model Peptide Ions. (Master's Thesis)