My research focuses on proteomics, lipidomics, biophysical chemistry and application and development of mass spectrometry, such as "label-free" nano-particle based biosensors and novel peptide/protein isolation and purification strategies. We are also investigating the structure(s) of model peptides in an effort to better describe folding/unfolding and structure of membrane and intrinsically disordered (IDP) proteins. Peptides take on very different 2?, 3? and 4? structure, which determine or influence bio-activity. In the presence of lipid vesicles peptides can exist as solution-phase species, "absorbed" on lipid bilayers or "inserted" (as a monomer or multimer) in lipid bilayers. By what mechanism do peptides interact with lipid membranes to affect these structural changes, how do peptide-lipid interactions promote self-assembly to form intermediates that eventually yield aggregates, i.e., amyloid fibrils, or how does metal ion coordination affect the structure of metalloproteins? Mass spectrometry-based experiments, hydrogen/deuterium (H/D) exchange, chemical 'foot-printing' and gas-phase (ion-molecule and ion-ion reaction chemistry) and solution-phase chemical modifications, have expanded our abilities to address such questions, and new instrumental approaches, esp. ion mobility spectrometry (IMS) combined with enhanced molecular dynamics simulations (MDS), have become standard tools for structural-mass spectrometry studies. Over the past several years we have either acquired or developed novel, next-generation IM-MS instruments that are redefining cutting-edge structural-mass spectrometry research as well as cutting-edge computational tools essential to carry out these studies. Our new laboratories in the Interdisciplinary Life Sciences Building (ILSB) provides exciting opportunities for collaborative, interdisciplinary research with chemical-biologists, biochemists and other chemists.
Academic Articles302
- McCabe, J. W., Shirzadeh, M., Walker, T. E., Lin, C., Jones, B. J., Wysocki, V. H., ... Russell, D. H. (2021). Variable-Temperature Electrospray Ionization for Temperature-Dependent Folding/Refolding Reactions of Proteins and Ligand Binding.. ANALYTICAL CHEMISTRY. 93(18), 6924-6931.
- Russell, D. H., & Clemmer, D. E. (2021). Editorial: Focus on Ionization Technologies Used in MS: Fundamentals and Applications, Honoring Dr. Sarah Trimpin, Recipient of the 2019 ASMS Biemann Medal.. Journal of The American Society for Mass Spectrometry. 32(3), 616-617.
- Moghadamchargari, Z., Shirzadeh, M., Liu, C., Schrecke, S., Packianathan, C., Russell, D. H., Zhao, M., & Laganowsky, A. (2021). Molecular assemblies of the catalytic domain of SOS with KRas and oncogenic mutants. Proc Natl Acad Sci U S A. 118(12), e2022403118-e2022403118.
- Mallis, C. S., Zheng, X., Qiu, X. i., McCabe, J. W., Shirzadeh, M., Lyu, J., Laganowsky, A., & Russell, D. H. (2020). Development of native MS capabilities on an extended mass range Q-TOF MS. International Journal of Mass Spectrometry. 458, 116451-116451.
- Schrecke, S., Zhu, Y., McCabe, J. W., Bartz, M., Packianathan, C., Zhao, M., ... Laganowsky, A. (2020). Selective regulation of human TRAAK channels by biologically active phospholipids. Nature Chemical Biology. 17(1), 89-95.
Chapters7
- May, J., & Russell, D. (2010). A Cryogenic-Temperature Ion Mobility Mass Spectrometer for Improved Ion Mobility Resolution. Ion Mobility Spectrometry - Mass Spectrometry. (pp. 137-151). CRC Press.
- Russell, D. H., Oriedo, J., & Solouki, T. (1996). Development of a Fourier-Transform Ion Cyclotron Resonance (FTICR) Mass Spectrometry Method for Studies of Metal Ion Excited States. Organometallic Ion Chemistry. (pp. 197-228). Springer Netherlands.
- Wang, Y., Yurttas, L., Dale, B. E., Russell, D. H., Kinsel, G. R., Preston, L. M., Wright, M. S., & Hayes, T. K. (1994). Matrix-Assisted Laser Desorption Mass Spectrometry To Monitor Synthesis And Folding of Manduca Sexta Eclosion Hormone And Its Analogs. Peptides. (pp. 265-267). Springer Netherlands.
- Russell, D. H., Fredeen, D. A., & Tecklenburg, R. E. (1989). Structure—Reactivity Relationships for Ionic Transition Metal Carbonyl Cluster Fragments. Gas Phase Inorganic Chemistry. (pp. 115-135). Springer US.
- Russell, D. H., & Castro, M. E. (1986). Desorption Ionization and Fourier Transform Mass Spectrometry for the Analysis of Large Biomolecules. THEORETICAL AND NUMERICAL UNSATURATED SOIL MECHANICS. Ion Formation from Organic Solids (IFOS III). (pp. 209-212). Springer Berlin Heidelberg.
Conference Papers76
- Liu, Y., Liu, W., Poltash, M., Russell, D., & Laganowsky, A. (2019). Native ion mobility mass spectrometry of membrane protein complexes. ACS National Meeting Book of Abstracts. 257,
- Russell, D. (2018). Native-ESI and ion mobility-mass spectrometry: Expanding MS-based structural biology through understanding of chemical fundamentals. ACS National Meeting Book of Abstracts. 255,
- Russell, D. (2017). Mapping peptide/protein conformation space: A challenge for IM-MS. ACS National Meeting Book of Abstracts. 253,
- Rogers, D., El-Baba, T., Shi, L., Khan, F., Kim, D., Hales, D., Russell, D., & Clemmer, D. (2016). Histidine-modified polyproline-13 explores the nature of two-state cooperativity. ACS National Meeting Book of Abstracts. 251,
- Russell, D. (2016). Mass spectrometry and structural biology: Ion mobility-mass spectrometry studies of water and water-mediated conformational preferences of peptides and proteins. ACS National Meeting Book of Abstracts. 251,
Patents5
- Mclean, J., Russell, D., Egan, T., Ugarov, M., & Schultz, A. (2010). Multiplex data acquisition modes for ion mobility-mass spectrometry.
- Mclean, J., Russell, D., Egan, T., Ugarov, M., & Schultz J. A., .. (2008). Multiplex data acquisition modes for ion mobility-mass spectrometry.
- Russell, D., & Mclean, J. (2007). Advanced optics for rapidly patterned laser profiles in analytical spectrometry.
- Mclean, J., Russell, D., & Schultz, A. (2006). Gas-phase purification of biomolecules by ion mobility for patterning microarrays and protein crystal growth.
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Principal Investigator3
- CHEM315 Fund. Of Quant. Analysis Instructor
- CHEM415 Analytical Chemistry Instructor
- CHEM491 Hnr-research Instructor
- CHEM491 Research Instructor
- CHEM491 Research: In-ab Instructor
- Kim, Doyong (2016-08). Monitorying the Desolvaiton of Ions and Create Candidate Structure for the Ions Detected by Ion Mobility-Mass Spectrometry Study by Molecular Dynamics Simulations. (Doctoral Dissertation)
- Servage, Kelly Anne (2016-08). Investigating the Effects of Dehydration on the Conformational Preferences of Biomolecules during the Final Stages of Electrospray Ionization. (Doctoral Dissertation)
- Lang, Phillip (2016-05). An Investigation of Polymer Encapsualted Gold Nanoparticles for Use in Analytical Methods for Proteomic Based Research. (Master's Thesis)
- Xiao, Chunying (2015-12). Effects of Charge States, Charge Sites and Side Chain Interactions on Conformational Preferences of a Series of Model Peptide Ions. (Master's Thesis)