Our laboratory focuses on systemic analysis of biochemical, molecular and biological functions of AGO family proteins (AGOs-mics) in genetically tractable Arabidopsis and economically important crops (i.e. rice). We'd like to identify the small RNAs, mRNA targets and protein components which associate with these AGOs. We will study protein/RNA and protein/protein interactions in these RISC assembly events. Our goal is to understand how these AGOs are functionally specialized or redundant corresponding to endogenous development cues and external environmental stimuli. Particularly, we'd like to learn how plants reprogram their gene expression through the small RNAs and AGOs to construct a new cellular niche in responses to environmental challenges and biotic stresses.
Another aspect of our research involves host/virus interaction. Plants take advantage of RNA silencing pathways to defend themselves from exogenous nucleic acid invaders (i.e. viruses). As an anti-host defense mechanism, viruses encode suppressors that can block RNA silencing responses. We have recently demonstrated that CMV 2b disables AGO1 cleavage activity to inhibit RNA silencing and to counter host defense. We are now extending our study to suppressors of several other viruses and the molecular mechanisms of their suppression.
- Rockefeller University - (New York, New York, United States), Postdoctoral Training 2008
- Ph.D. in , Cornell University - (Ithaca, New York, United States) 2003
- M.S. in , Auburn University - (Auburn, Alabama, United States) 1999
- Li, Y., Sun, D. i., Ma, Z., Yamaguchi, K., Wang, L., Zhong, S., ... Zhang, X. (2020). Degradation of SERRATE via ubiquitin-independent 20S proteasome to survey RNA metabolism. Nature Plants. 6(8), 970-982.
- Nagashima, Y., Ma, Z., Zhang, X., von Schaewen, A., & Koiwa, H. (2020). Lack of endoplasmic reticulum quality control (ERQC) promotes tonoplast (TP) targeting of KORRIGAN 1 (KOR1). Plant Signaling & Behavior. 15(4), 1744348-1744348.
- Nagashima, Y., Ma, Z., Liu, X., Qian, X., Zhang, X., von Schaewen, A., & Koiwa, H. (2020). Multiple Quality Control Mechanisms in the ER and TGN Determine Subcellular Dynamics and Salt-Stress Tolerance Function of KORRIGAN1.. The Plant Cell. 32(2), 470-485.
- Hu, T., Huang, C., He, Y., Castillo-González, C., Gui, X., Wang, Y., Zhang, X., & Zhou, X. (2019). βC1 protein encoded in geminivirus satellite concertedly targets MKK2 and MPK4 to counter host defense. PLoS Pathogens. 15(4), e1007728-e1007728.
- Wang, Z., Wang, M., Wang, T., Zhang, Y., & Zhang, X. (2018). Genome-wide probing RNA structure with the modified DMS-MaPseq in Arabidopsis. Methods. 155, 30-40.
- CAREER: Arabidopsis Argonaute10-protein interactome awarded by National Science Foundation 2013 - 2019