We combine approaches in molecular genetics, structural biology, biochemistry, proteomics, and bioinformatics to study the amazing RNA biology of trypanosome parasites. One research line is on an RNA editing process by uridine insertion and deletion that creates amino acid coding triplets in most mRNAs. Yet a single error in the U-changes yields a frame-shift. Trypanosomes split from other eukaryotic lineages over a hundred million years ago, yet this editing has analogies with RNAi, CRISPR/Cas9, mRNA splicing and other systems directed by small non-coding RNAs (ncRNAs).
- Kumar, V., Doharey, P. K., Gulati, S., Meehan, J., Martinez, M. G., Hughes, K., Mooers, B., & Cruz-Reyes, J. (2019). Protein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes. PLoS ONE. 14(4), e0211525-e0211525.
- Kumar, V., Doharey, P., Gulati, S., Meehan, J., Martinez, M., Hughes, K., Mooers, B., & Cruz-Reyes, J. (2019). Protein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes.
- Cruz-Reyes, J., Mooers, B., Doharey, P. K., Meehan, J., & Gulati, S. (2018). Dynamic RNA holo-editosomes with subcomplex variants: Insights into the control of trypanosome editing. Wiley Interdisciplinary Reviews: RNA. 9(6), e1502-e1502.
- Kumar, V., Madina, B. R., Gulati, S., Vashisht, A. A., Kanyumbu, C., Pieters, B., ... Cruz-Reyes, J. (2016). REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes. JOURNAL OF BIOLOGICAL CHEMISTRY. 291(11), 5753-5764.
- Cruz-Reyes, J., Mooers, B., Abu-Adas, Z., Kumar, V., & Gulati, S. (2016). DEAH-RHA helicase•Znf cofactor systems in kinetoplastid RNA editing and evolutionarily distant RNA processes.. RNA Dis. 3(2),
- Cruz-Reyes, J., & Hernandez, A. (2007). Protein-Protein and RNA-Protein Interactions in U-Insertion/Deletion RNA Editing Complexes. RNA and DNA Editing: Molecular Mechanisms and Their Integration into Biological Systems. (pp. 71-98). John Wiley %26 Sons, Inc..
- Kumar, Vikas (2016-05). The Function of a Central RNA Helicase RNP in Editosomes of Trypanosomes. (Doctoral Dissertation)
- Hernandez, Alfredo J. (2010-12). RNA Editing in Trypanosomes: Substrate Recognition and its Integration to RNA Metabolism. (Doctoral Dissertation)