Live bioreactor for synthetic biology
The lab is developing live bioreactors to synthesize products of commercial value. The system we are developing is capable of resisting contamination, and withstanding harsh conditions. We are translating the technology developed for potential industrial usages.
The biogenesis of a cilium/flagellum
Our lab is interested in the conceptual frameworks that govern organelle biogenesis and the corresponding regulations. The current main research effort in our lab is to understand. Cilia and flagella are microtubule-based appendages extending from the basal body of almost all eukaryotic cells, and are classified as either motile or primary. Motile cilia or flagella such as Chlamydomonas flagella, sperm flagella and respiratory tract epithelial cell cilia are responsible for movement or generation of fluid flow. In contrast, primary cilia are non-motile organelles that are critically involved in visual, olfactory and auditory signal transduction and play key roles in regulation of gene expression, development and animal behavior. Ciliary defects are linked to ciliopathies such as polycystic kidney disease, nephronophthisis, retinal degeneration, situs inversus, hydrocephalus, polydactyly and obesity. Our lab uses a combination of biochemistry, cell biology, and genetics approaches to understand the principles of ciliogenesis and its regulation.
Flagellar axoneme structure and motility
The waveform of cilia is conserved, no matter whether the cilia are on green algae Chlamydomonas or mammalian epithelia found in the airways, the uterus and fallopian tubes, the efferent ducts of the testes, and the ventricular system of the brain. These motile cilia beat with a conserved planar asymmetrical waveform. We are beginning to learn how the asymmetry of the waveform is established and the mutant analyses are underway.
- Yale University - (New Haven, Connecticut, United States), Postdoctoral Training 2005
- Ph.D. in Genetics, Institute of Microbiology - (Beijing, China) 1999
- M.S. in Microbiology, Shandong University - (Jinan, Shandong, China) 1996
- B.S. in Microbiology, Shandong University - (Jinan, Shandong, China) 1993
- Wu, B., Chun, E., Xie, R., Knox, G. W., Gu, M., & Qin, H. (2022). Real-Time Feeding Behavior Monitoring by Electrical Penetration Graph Rapidly Reveals Host Plant Susceptibility to Crapemyrtle Bark Scale (Hemiptera: Eriococcidae). Insects. 13(6), 495-495.
- Ishikawa, H., Tian, J. L., Yu, J. E., Marshall, W. F., & Qin, H. (2022). Biosynthesis of Linear Protein Nanoarrays Using the Flagellar Axoneme. ACS Synthetic Biology. 11(4), 1454-1465.
- Xie, R., Wu, B., Gu, M., & Qin, H. (2022). Life table construction for crapemyrtle bark scale (Acanthococcus lagerstroemiae): the effect of different plant nutrient conditions on insect performance.. Sci Rep. 12(1), 11472.
- Ma, R., Hendel, N. L., Marshall, W. F., & Qin, H. (2020). Speed and Diffusion of Kinesin-2 Are Competing Limiting Factors in Flagellar Length-Control Model. Biophysical Journal. 118(11), 2790-2800.
- Finetti, F., Pan, J., Qin, H., & Delaval, B. (2020). Editorial: Dissecting the Intraflagellar Transport System in Physiology and Disease: Cilia-Related and -Unrelated Roles. Frontiers in Cell and Developmental Biology. 8, 615588.
- Ishikawa, H., Yu, J., Tian, J., Tang, S., Qin, H., & Marshall, W. F. (2019). Cell-based biosynthesis of linear protein nanoarrays. Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI. 10893, 108930f-108930f-16.
- Ishikawa, H., Ide, T., Yagi, T., Jiang, X., Yanagisawa, H., Sasaki, H., ... Marshall, W. (2012). TTC26/DYF13 undergoes intraflagellar transport and is required for cilia/flagella formation and transport of inner dynein components into flagella.. MOLECULAR BIOLOGY OF THE CELL. 23,
- Richey, E., & Qin, H. (2011). IFT46, IFT52, and IFT88 play different roles in IFT Complex B assembly, stability, and apical basal body localization in Chlamydomonas reinhardtii.. MOLECULAR BIOLOGY OF THE CELL. 22,
- Wang, Z., Qin, H., Diener, D., & Rosenbaum, J. (2006). Knockdown of IFT27 Lead to Severe Cell Cycle Defect in Chlamydomonas. MOLECULAR BIOLOGY OF THE CELL. 17,
- Bae, Y., Knobel, K., Qin, H., Hu, J., Rosenbaum, J., & Barr, M. (2006). Mechanisms of Polycystin Ciliary Localization in C-elegans. MOLECULAR BIOLOGY OF THE CELL. 17,
Repository Documents / Preprints2
- Ishikawa, H., Tian, J. L., Yu, J. E., Marshall, W. F., & Qin, H. (2021). Biosynthesis of linear protein nanoarrays using the flagellar axoneme.
- Liu, Y. i., Visetsouk, M., Mynlieff, M., Qin, H., Lechtreck, K. F., & Yang, P. (2017). H+- and Na+-elicited swift changes of the microtubule system in the biflagellated green alga Chlamydomonas.
- Jiang, Xue (2017-05). Eukaryotic Flagellar Assembly: Insights Learned from Intraflagellar Transport (IFT) and Small GTPase ARL3. (Doctoral Dissertation)
- Silva, David (2012-12). Functional Analysis of an Integrated GTPase Regulating the Cellular Pool and Distribution Profile of Intraflagellar Transport Particles in Chlamydomonas Reinhardtii. (Master's Thesis)
- Richey, Elizabeth (2012-12). Probing the Roles that Intraflagellar Transport B Protiens Play on Stability, Assembly, and Localization of Complex B in Chlamydomonas ReinhardtII. (Master's Thesis)