We are taking biochemical, molecular genetic and cytological approaches to study the structure, function and maintenance of telomeres. Telomeres are higher order nucleoprotein complexes that cap the ends of eukaryotic chromosomes and play essential roles in conferring genome stability and cell proliferation capacity. The protective cap of the telomere is comprised of specific telomere binding proteins that regulate the length of telomeric DNA tract and allow the cell distinguish the chromosome terminus from a double-strand break. Telomeric DNA is synthesized by the action of telomerase, an unusual reverse transcriptase that replenishes telomeric DNA lost as a consequence of replication by conventional DNA polymerases. We have developed the genetically tractable flowering plant Arabidopsis thaliana as a model system for studying telomeres in higher eukaryotes. With its sequenced genome, abundant genetic and transgenic tools, and extraordinarily high tolerance to genome instability, Arabidopsis has proven to be an excellent model for investigating fundamental processes in telomere biology. Current studies focus on defining the function and molecular evolution of telomere capping proteins and components of the telomerase ribonucleoprotein complex.
- University of California, Berkeley - (Berkeley, California, United States), Postdoctoral Training 1991
- Ph.D. in , University of Alabama at Birmingham - (Birmingham, Alabama, United States) 1987
- B.S. in , Auburn University - (Auburn, Alabama, United States) 1982
- Abdulkina, L. R., Kobayashi, C., Lovell, J. T., Chastukhina, I. B., Aklilu, B. B., Agabekian, I. A., ... Shakirov, E. V. (2019). Components of the ribosome biogenesis pathway underlie establishment of telomere length set point in Arabidopsis. Nature Communications. 10(1), 5479.
- Song, J., Logeswaran, D., Castillo-Gonzalez, C., Li, Y., Bose, S., Aklilu, B. B., ... Shippen, D. E. (2019). The conserved structure of plant telomerase RNA provides the missing link for an evolutionary pathway from ciliates to humans. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES. 116(49), 24542-24550.
- Barcenilla, B. B., & Shippen, D. E. (2019). Back to the future: The intimate and evolving connection between telomere-related factors and genotoxic stress. JOURNAL OF BIOLOGICAL CHEMISTRY. 294(40), 14803-14813.
- Kobayashi, C. R., Castillo-Gonzalez, C., Survotseva, Y., Canal, E., Nelson, A., & Shippen, D. E. (2019). Recent emergence and extinction of the protection of telomeres 1c gene in Arabidopsis thaliana. Plant Cell Rep. 38(9), 1081-1097.
- Xie, X., & Shippen, D. E. (2018). DDM1 guards against telomere truncation in Arabidopsis. Plant Cell Rep. 37(3), 501-513.
- Nelson, A., Beilstein, M. A., & Shippen, D. E. (2014). Plant telomeres and telomerase. Molecular Biology. (pp. 25-49). Springer New York.
- Lamb, J. C., Shakirov, E. V., & Shippen, D. E. (2012). Plant telomeres. Plant Cytogenetics: Genome Structure and Chromosome Function. (pp. 143-191). Springer New York.
- Xie, Xiaoyuan (2017-05). Novel Players in Telomere Maintenance and Beyond. (Doctoral Dissertation)
- Xu, Hengyi (2015-05). Characterization of the Arabidopsis Telomerase Negative Regulator: A TE-Containing lncRNA TER2- Defining Its Role in and beyond Telomere Biology. (Doctoral Dissertation)
- Renfrew, Kyle Brayton (2014-12). Telomere Dynamics in Arabidopsis thaliana. (Doctoral Dissertation)
- Boltz, Kara A. (2013-12). Telomere Regulation in Arabidopsis thaliana by the CST Capping Complex and DNA Damage Response Proteins. (Doctoral Dissertation)
- Leehy, Katherine (2013-08). Dissection of the telomere complex CST in Arabidopsis thaliana. (Doctoral Dissertation)