- Regents Professor and Head, Microbial Pathogenesis and Immunology, College of Medicine
Our laboratory works with the obligate intracellular bacterial pathogen, Coxiella burnetii, the etiologic agent of Q fever and a category B biothreat agent. The long-term goal of this research is to understand the molecular pathogenic mechanisms involved in the host-pathogen interaction. To accomplish this broad goal, project in the lab are designed to test the molecular mechanisms employed by both the host and pathogen. Current pathogen studies include 1) broad survey of proteins secreted via a type 4 secretion system (T4SS) followed by determination of essentiality of each substrate for virulence and detailed analysis of mechanism of host modulation 2) survey of essential virulence loci identified by specific mutant screens, and 3) definition of the relative virulence of phylogenetically distinct isolate groups.
- Uniformed Services University of the Health Sciences - (Bethesda, Maryland, United States), Postdoctoral Training 1990
- Ph.D. in Microbiology, Washington State University - (Pullman, Washington, United States) 1986
- M.S. in Biology, Washington State University - (Pullman, Washington, United States) 1983
- B.A. in Zoology and Chemistry, Miami University - (Oxford, Ohio, United States) 1976
Academic Articles102
- Gregory, A. E., van Schaik, E. J., Fratzke, A. P., Russell-Lodrigue, K. E., Farris, C. M., & Samuel, J. E. (2021). Soluble antigens derived from Coxiella burnetii elicit protective immunity in three animal models without inducing hypersensitivity. Cell Reports Medicine. 2(12), 100461-100461.
- Fratzke, A. P., Gregory, A. E., van Schaik, E. J., & Samuel, J. E. (2021). Coxiella burnetii Whole Cell Vaccine Produces a Th1 Delayed-Type Hypersensitivity Response in a Novel Sensitized Mouse Model. Frontiers in Immunology. 12, 754712.
- Ouyang, Y., Chen, D., Fu, Y., Shi, W., Provin, T., Han, A., ... Zhou, J. (2021). Direct cell extraction from fresh and stored soil samples: Impact on microbial viability and community compositions. Soil Biology and Biochemistry. 155, 108178-108178.
- Fratzke, A. P., Jan, S., Felgner, J., Liang, L. i., Nakajima, R., Jasinskas, A., ... Gregory, A. E. (2021). Subunit Vaccines Using TLR Triagonist Combination Adjuvants Provide Protection Against Coxiella burnetii While Minimizing Reactogenic Responses.. Front Immunol. 12, 653092.
- Chen, C., van Schaik, E. J., Gregory, A. E., Vigil, A., Felgner, P. L., Hendrix, L. R., Faris, R., & Samuel, J. E. (2019). Chemokine Receptor 7 Is Essential for Coxiella burnetii Whole-Cell Vaccine-Induced Cellular Immunity but Dispensable for Vaccine-Mediated Protective Immunity.. J Infect Dis. 220(4), 624-634.
Chapters2
- Toman, R., Heinzen, R. A., Samuel, J. E., & Mege, J. (2012). Coxiella burnetii: Recent Advances and New Perspectives in Research of the Q Fever Bacterium Preface. MATHEMATICAL MODELLING IN EXPERIMENTAL NUTRITION. COXIELLA BURNETII: RECENT ADVANCES AND NEW PERSPECTIVES IN RESEARCH OF THE Q FEVER BACTERIUM. V-XI.
Conference Papers2
- TESH, V. L., SAMUEL, J. E., BURRIS, J. A., OWENS, J. W., TAYLOR, F. B., & SIEGLER, R. L. (1994). QUANTITATION AND LOCALIZATION OF SHIGA TOXIN SHIGA-LIKE TOXIN-BINDING GLYCOLIPID RECEPTORS IN HUMAN AND BABOON TISSUES. RECENT ADVANCES IN VEROCYTOTOXIN-PRODUCING ESCHERICHIA COLI INFECTIONS. 1072, 189-192.
- Frazier, M. E., Mallavia, L. P., Samuel, J. E., & Baca, O. G. (1990). DNA probes for the identification of Coxiella burnetti strains.. Ann N Y Acad Sci. 590(1 Rickettsiolog), 445-458.
Patents1
Principal Investigator5
- BICH491 Hnr-research Instructor
- BICH491 Research Instructor
- BIOT684 Directed Prof Internship Instructor
- BIOT684 Directed Prof Intrn Instructor
- BIOT684 Directed Prof Intrn: In-ab Instructor
- Rowin, Kristina D (2014-12). Identifying Coxiella burnetii Genes Essential for Subversion of the Host Immune System. (Master's Thesis)
- Weber, Mary (2014-05). Identification of C. burnetii Type IV Secretion Substrates Required for Intracellular Replication and Coxiella-Containing Vacuole Formation. (Doctoral Dissertation)
- Russell-Lodrigue, Kasi Elizabeth (2006-12). An inhalation model of acute Q fever in guinea pigs. (Doctoral Dissertation)
- Hong, Priscilla Christine (2005-08). Utilization of the persistent nature of Brucella in the development of live vaccines. (Doctoral Dissertation)