Clark, Karl
individual record
Associate Professor of Precision Gene Editing
Positions:
- Associate Professor, Animal Science, College of Agriculture and Life Sciences
overview
My research is built upon my interest and long history of technology development and genome engineering. I collaborate with a large array of scientists with whom I work to develop new methods of gene editing. At Texas A&M my primary interests are applying gene editing technologies to improve agriculture by improving animal welfare and production in response to new environmental challenges including climate change and pathogens. In addition to work in agricultural species, my lab uses the zebrafish model to aid in development of new genome engineering technology. We apply these molecular and genetic tools to dissect and discover the interactions between genes and the environment that affect both short and long-term changes in physiology and behavior. We have developed zebrafish assays and genetic tools to identify modifiers of hypothalamic-pituitary-adrenal (interrenal) stress responses looking at both rapid (non-genomic) and delayed (genomic) responses to stressors.
education and training
- Ph.D. in Molecular, Cellular, Developmental Biology, and Genetics, University of Minnesota - (Minneapolis, Minnesota, United States) 2003
- B.S. in Biochemistry and Molecular Biology, University of Wisconsin-Eau Claire - (Eau Claire, Wisconsin, United States) 1994
selected publications
Academic Articles101
- Lee, H. B., Shams, S., Dang Thi, V. H., Boyum, G. E., Modhurima, R., Hall, E. M., ... Clark, K. J. (2024). Key HPI axis receptors facilitate light adaptive behavior in larval zebrafish.. Scientific Reports. 14(1), 7759.
- Wilke, M., Wick, M., Schwab, T. L., Starosta, R. T., Clark, K. J., Connolly, H. M., & Klee, E. W. (2024). Nuclear Abnormalities in LMNA p.(Glu2Lys) Variant Segregating with LMNA-Associated Cardiocutaneous Progeria Syndrome.. Genes. 15(1), 112-112.
- Lasho, T. L., Finke, C. M., Fernandez, J. A., Schmitz, C. T., Clark, K. J., Wahlestedt, C., ... Binder, M. (2023). Targeting Oncogenic Enhancers in ASXL1-Mutant Chronic Myelomonocytic Leukemia. Blood. 142(Supplement 1), 1413-1413.
- Morales-Rosado, J. A., Schwab, T. L., Macklin-Mantia, S. K., Foley, A. R., Pinto E Vairo, F., Pehlivan, D., ... Klee, E. W. (2023). Bi-allelic variants in HMGCR cause an autosomal-recessive progressive limb-girdle muscular dystrophy.. American Journal of Human Genetics. 110(6), 989-997.
- Kar, B., Thulung, L. R., Castillo, S. R., Sabharwal, A., Clark, K., & Ekker, S. (2023). 34 Gene Editing/Gene Therapies: ENHANCED MITOCHONDRIAL BASE EDITING SYSTEM FOR NEAR COMPLETE MITOCHONDRIAL GENOME EDITING IN HUMAN PRIMARY CELLS SUITABLE FOR DISEASE MODELING AND FOR POTENTIAL THERAPEUTIC APPLICATIONS. Cytotherapy. 25(6), s25-S25.
Chapters9
- Lee, H. B., Modhurima, R., Heeren, A. A., & Clark, K. J. (2020). Chapter 16 Designer mutants for behavioral genetics. Behavioral and Neural Genetics of Zebrafish. 263-278. Elsevier.
- Kim, J., Clark, K., Barton, C., Tanguay, R., & Moulton, H. (2018). A Novel Zebrafish Model for Assessing In Vivo Delivery of Morpholino Oligomers.. Methods in molecular biology (Clifton, N.J.). Exon Skipping and Inclusion Therapies. 293-306. Springer Nature.
- Khoury, L., Campbell, J. M., & Clark, K. J. (2018). Chapter 17 The Transition of Zebrafish Functional Genetics From Random Mutagenesis to Targeted Integration. Molecular-Genetic and Statistical Techniques for Behavioral and Neural Research. 401-416. Elsevier.
- Ata, H., Clark, K. J., & Ekker, S. C. (2016). The zebrafish genome editing toolkit.. Methods in Cell Biology. The Zebrafish - Genetics, Genomics, and Transcriptomics. 149-170. Elsevier.
- Clark, K. J., & Ekker, S. C. (2013). Zebrafish. Brenner's Encyclopedia of Genetics. 396-398. Elsevier.
Repository Documents / Preprints5
- Castillo, S. R., Simone, B. W., Clark, K. J., Devaux, P., & Ekker, S. C. (2024). Unconstrained Precision Mitochondrial Genome Editing with DdCBEs..
- Lee, H. B., Thi, V., Boyum, G. E., Modhurima, R., Hall, E. M., Green, I. K., ... Clark, K. J. (2022). The canonical HPA axis contributes to locomotion during photoadaptation but is not required.
- Simone, B. W., Lee, H. B., Daby, C. L., Restrepo-Castillo, S., Martnez-Glvez, G., Ata, H., ... Ekker, S. C. (2021). Chimeric RNA:DNA Donorguide Improves HDR in vitro and in vivo.
- Sabharwal, A., Kar, B., Restrepo-Castillo, S., Holmberg, S. R., Kendall, B. L., Cotter, R. P., ... Ekker, S. C. (2021). The FusX TALE Base Editor (FusXTBE) for rapid mitochondrial DNA programming of human cells in vitro and zebrafish disease models in vivo.
- Almeida, M. P., Welker, J. M., Ekker, S. C., Clark, K. J., Essner, J. J., & McGrail, M. (2020). Endogenous zebrafish proneural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration.
patents
Patents8
- Clark, K. J., Ekker, S. C., Essner, J., Welker, J. M., de Almeida, M. P., Wierson, W. A., & McGrail, M. (2020). Materials and methods for efficient targeted knock in or gene replacement.
- Ekker, S. C., Clark, K. J., Campbell, J. M., & Ma, C. (2018). Methods and materials for assembling nucleic acid constructs.
- Hackett, P. B., Clark, K. J., Cui, Z., Dupuy, A. J., Geurts, A. M., & Liu, G. (2012). Transposon system and methods of use.
- Fahrenkrug, S. C., Clark, K. J., Carlson, D. F., & Leaver, M. J. (2010). Plaice dna transposon system.
recent teaching activities
- ANSC491 Hnr-research Instructor
- ANSC491 Research Instructor
- ANSC681 Seminar Instructor
- ANSC691 Research Instructor
- ANSC691 Research: In-ab Instructor
Email
karl.clark@ag.tamu.edu
First Name
Karl
Last Name
Clark
