The molecular mechanism of the metal specificity to superoxide dismutase from human pathogen C. difficile (SODcd) was investigated by X-ray crystallography, spectroscopy, SOD activity assay, electrochemistry, and DFT calculations, and the results indicate that the cognate metal characters tuned by the metal micro-environment dominate the metal specificity of the SODcd.
- Clostridium Difficile
- Superoxide Dismutase
- Electron Spin Resonance Spectroscopy
- Protein Structure, Tertiary