My lab uses X-ray crystallography to better understand the relationship between proteins and ligands. Tiny differences in the structure of a molecule can radically change the interaction between a protein and ligand and we are only begining to understand how many factors play a role in this interaction. By manipulating the individual components of a compound it is possible to create a chemical that binds to the protein better than the natural substrate, and prevent the natural reaction from occurring. This is the basis for rational drug design. Our efforts have lead us to collaborations with other labs and scientists in many disciplines as our approach to directed compound design has applications not only in basic research but also in pesticide development, health research and clinical research.
- Ballinger, E., Mosior, J., Hartman, T., Burns-Huang, K., Gold, B., Morris, R., ... Nathan, C. (2019). Opposing reactions in coenzyme A metabolism sensitize Mycobacterium tuberculosis to enzyme inhibition. Science (New York, N.Y.). 363(6426), eaau8959-+.
- Salazar, A. J., Sherekar, M., Tsai, J., & Sacchettini, J. C. (2019). R pyocin tail fiber structure reveals a receptor-binding domain with a lectin fold. PLoS ONE. 14(2), e0211432-e0211432.
- Miller, B. K., Hughes, R., Ligon, L. S., Rigel, N. W., Malik, S., Anjuwon-Foster, B. R., Sacchettini, J. C., & Braunstein, M. (2019). Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathway. Elife. 8, e40063.
- Rittershaus, E., Baek, S., Krieger, I. V., Nelson, S. J., Cheng, Y., Nambi, S., ... Sassetti, C. M. (2018). A Lysine Acetyltransferase Contributes to the Metabolic Adaptation to Hypoxia in Mycobacterium tuberculosis. Cell Chemical Biology. 25(12), 1495-1505.e3.
- Singh, S. B., Odingo, J., Bailey, M. A., Sunde, B., Korkegian, A., O’Malley, T., ... Parish, T. (2018). Identification of cyclic hexapeptides natural products with inhibitory potency against Mycobacterium tuberculosis. BMC Research Notes. 11(1), 416.
- Musa, T. L., Ioerger, T. R., & Sacchettini, J. C. (2009). THE TUBERCULOSIS STRUCTURAL GENOMICS CONSORTIUM: A STRUCTURAL GENOMICS APPROACH TO DRUG DISCOVERY. Advances in protein chemistry and structural biology. STRUCTURAL GENOMICS, PART C. (pp. 41-76).
- Lücke, C., Fushman, D., Ludwig, C., Hamilton, J. A., Sacchettini, J. C., & Rüterjans, H. (1999). A comparative study of the backbone dynamics of two closely related lipid binding proteins: Bovine heart fatty acid binding protein and porcine ileal lipid binding protein. Lipid Binding Proteins within Molecular and Cellular Biochemistry. (pp. 109-121). Springer Us.
- Scapin, G., Young, A., Kromminga, A., Veerkamp, J. H., Gordon, J. I., & Sacchettini, J. C. (1993). High resolution X-ray studies of mammalian intestinal and muscle fatty acid-binding proteins provide an opportunity for defining the chemical nature of fatty acid: protein interactions. Cellular Fatty Acid-Binding Proteins II. (pp. 3-13). Springer Us.
- Cistola, D. P., Sacchettini, J. C., & Gordon, J. I. (1990). 13C NMR studies of fatty acid-protein interactions: comparison of homologous fatty acid-binding proteins produced in the intestinal epithelium. Cellular Fatty Acid-binding Proteins. (pp. 101-110). Springer Us.
- Sacchettini, J. C., Banaszak, L. J., & Gordon, J. I. (1990). Expression of rat intestinal fatty acid binding protein in E. coli and its subsequent structural analysis: a model system for studying the molecular details of fatty acid-protein interaction. Cellular Fatty Acid-binding Proteins. (pp. 81-93). Springer Us.
- Gopal, K., Romo, T. D., Sacchettini, J. C., & Ioerger, T. R. (2004). Weighting features to recognize 3D patterns of electron density in X-ray protein crystallography. 255-265.
- Savva, C. G., Arulandu, A., Sacchettini, J., & Holzenburg, A. (2003). Electron microscopy and single particle analysis of a mycobacterial preprotein translocation, ATPase. MICROSCOPY AND MICROANALYSIS. 9(SUPPL. 2), 1364-1365.
- Sun, J., Sridharan, S., Sacchettini, J., Savva, C., & Holzenburg, A. (2003). TEM studies of IniA from Mycobacterium tuberculosis. MICROSCOPY AND MICROANALYSIS. 9(SUPPL. 2), 1418-1419.
- Gopal, K., Pai, R., Ioerger, T. R., Romo, T. D., & Sacchettini, J. C. (2003). TEXTALTM: Artificial Intelligence Techniques for Automated Protein Structure Determination.. 93-100.
- Tao, Y. u., Ioerger, T. R., & Sacchettini, J. C. (2002). Extracting Fractal Features for Analyzing Protein Structure. Proceedings - International Conference on Pattern Recognition. 2(2), 482-485.
- Reddy, M., Sacchettini, J. C., Zhou, N. E., & Mccutchen, B. F. (2019). Compositions and methods for inhibition of mycobacteria.
- Sacchettini, J. C., Miller, M. W., Wallis, D., Zhou, N. E., & Fossum, T. W. (2019). Compositions and methods for drug sensitization of parasites.
- Sacchettini, J., Zhou, N., Baker, D., Maxwell, S., & Wallis, D. (2018). Compositions and methods for drug-sensitization or inhibition of a cancer cell.
- Rao, J., Cirillo, J., Xie, H., & Sacchettini, J. (2017). ?-lactamase substrates and methods of their use for the diagnosis of tuberculosis.
- Rao, J., Cirillo, J. D., Xie, H., & Sacchettini, J. C. (2017). β-lactamase substrates and methods of their use for the diagnosis of tuberculosis.