My lab uses X-ray crystallography to better understand the relationship between proteins and ligands. Tiny differences in the structure of a molecule can radically change the interaction between a protein and ligand and we are only begining to understand how many factors play a role in this interaction. By manipulating the individual components of a compound it is possible to create a chemical that binds to the protein better than the natural substrate, and prevent the natural reaction from occurring. This is the basis for rational drug design. Our efforts have lead us to collaborations with other labs and scientists in many disciplines as our approach to directed compound design has applications not only in basic research but also in pesticide development, health research and clinical research.
- Singh, S. B., Odingo, J., Bailey, M. A., Sunde, B., Korkegian, A., O’Malley, T., ... Parish, T. (2018). Identification of cyclic hexapeptides natural products with inhibitory potency against Mycobacterium tuberculosis. BMC Research Notes. 11(1), 416.
- Ellenbarger, J. F., Krieger, I. V., Huang, H., Gómez-Coca, S., Ioerger, T. R., Sacchettini, J. C., Wheeler, S. E., & Dunbar, K. R. (2018). Anion-π Interactions in Computer-Aided Drug Design: Modeling the Inhibition of Malate Synthase by Phenyl-Diketo Acids.. J Chem Inf Model. 58(10), 2085-2091.
- Ganley, J. G., Carr, G., Ioerger, T. R., Sacchettini, J. C., Clardy, J., & Derbyshire, E. R. (2018). Discovery of Antimicrobial Lipodepsipeptides Produced by a Serratia sp. within Mosquito Microbiomes. Chembiochem : a European journal of chemical biology. 19(15), 1590-1594.
- Ganley, J. G., Carr, G., Ioerger, T. R., Sacchettini, J. C., Clardy, J., & Derbyshire, E. R. (2018). Front Cover: Discovery of Antimicrobial Lipodepsipeptides Produced by a Serratia sp. within Mosquito Microbiomes (ChemBioChem 15/2018). Chembiochem : a European journal of chemical biology. 19(15), 1570-1570.
- Blanc, L., Sarathy, J. P., Cabrera, N. A., O’Brien, P., Dias-Freedman, I., Mina, M., ... Dartois, V. (2018). Impact of immunopathology on the antituberculous activity of pyrazinamidePyrazinamide sterilizes necrotic TB lesions. The Journal of Experimental Medicine. 215(8), 1975-1986.
- Musa, T. L., Ioerger, T. R., & Sacchettini, J. C. (2009). THE TUBERCULOSIS STRUCTURAL GENOMICS CONSORTIUM: A STRUCTURAL GENOMICS APPROACH TO DRUG DISCOVERY. Advances in protein chemistry and structural biology. STRUCTURAL GENOMICS, PART C. (pp. 41-76).
- Lücke, C., Fushman, D., Ludwig, C., Hamilton, J. A., Sacchettini, J. C., & Rüterjans, H. (1999). A comparative study of the backbone dynamics of two closely related lipid binding proteins: Bovine heart fatty acid binding protein and porcine ileal lipid binding protein. Lipid Binding Proteins within Molecular and Cellular Biochemistry. (pp. 109-121). Springer Us.
- Scapin, G., Young, A., Kromminga, A., Veerkamp, J. H., Gordon, J. I., & Sacchettini, J. C. (1993). High resolution X-ray studies of mammalian intestinal and muscle fatty acid-binding proteins provide an opportunity for defining the chemical nature of fatty acid: protein interactions. Cellular Fatty Acid-Binding Proteins II. (pp. 3-13). Springer Us.
- Cistola, D. P., Sacchettini, J. C., & Gordon, J. I. (1990). 13C NMR studies of fatty acid-protein interactions: comparison of homologous fatty acid-binding proteins produced in the intestinal epithelium. Cellular Fatty Acid-binding Proteins. (pp. 101-110). Springer Us.
- Sacchettini, J. C., Banaszak, L. J., & Gordon, J. I. (1990). Expression of rat intestinal fatty acid binding protein in E. coli and its subsequent structural analysis: a model system for studying the molecular details of fatty acid-protein interaction. Cellular Fatty Acid-binding Proteins. (pp. 81-93). Springer Us.
- Lieberman, J. M., Marks, W. H., Cohn, S., Jaicks, R., Woode, L., Sacchettini, J., ... Burns, G. (1998). Organ Failure, Infection, and the Systemic Inflammatory Response Syndrome Are Associated with Elevated Levels of Urinary Intestinal Fatty Acid Binding Protein. J Trauma Acute Care Surg. 45(5), 900-906..
- Purkey, H. E., Lashuel, H. A., Peterson, S. A., Baures, P. W., Klabunde, T., Sacchettini, J. C., & Kelly, J. W. (1998). Inhibiting transthyretin amyloid formation with small molecules.. ACS Photonics. 216, U219-U219.
- Sacchettini, J. C. (1997). Combatting drug resistant tuberculosis.. ACS Photonics. 214, 7-CARB.
- Zhang, F. L., Lucke, C., Baier, L. J., Sacchettini, J. C., & Hamilton, J. A. (1997). Comparison of T54 allele with normal A54 allele of human intestinal fatty acid binding protein.. Biophys J. 72(2), MP294-MP294.
- Xu, Y., Eads, J., Sacchettini, J. C., & Grubmeyer, C. (1996). Catalytic base in hypoxanthine-guanine phosphoribosyltransferase (HGPRTase).. ACS Photonics. 212, 45-BIOL.
- Sacchettini, J. C., Miller, M. W., Wallis, D., Zhou, N. E., & Fossum, T. W. (2019). Compositions and methods for drug sensitization of parasites.
- Reddy, M., Sacchettini, J. C., Zhou, N. E., & Mccutchen, B. F. (2019). Compositions and methods for inhibition of mycobacteria.
- Sacchettini, J., Zhou, N., Baker, D., Maxwell, S., & Wallis, D. (2018). Compositions and methods for drug-sensitization or inhibition of a cancer cell.
- Rao, J., Cirillo, J., Xie, H., & Sacchettini, J. (2017). ?-lactamase substrates and methods of their use for the diagnosis of tuberculosis.
- Sacchettini, J., Zhou, N., Baker, D., Maxwell, S., & Wallis, D. (2017). Compositions and methods for drug-sensitization or inhibition of a cancer cell.
- Liu, Zhen (2013-12). Understanding and Targeting Lipid Metabolism of Mycobacterium tuberculosis. (Doctoral Dissertation)
- Tsai, Han-Chun (2013-08). Structural Study of Lipid-binding Proteins. (Doctoral Dissertation)
- Mire, Joseph Andrew (2013-08). Application of Structure Activity Relationships of the Mycobacterium Tuberculosis Beta-Lactamase (BlaC) and the New Delhi Metallo-Beta-Lactamase (NDM-1) to Combating Beta-Lactamase Mediated Drug Resistance. (Doctoral Dissertation)
- Kuznetsov, Vladimir 1973- (2011-08). Structural Studies of Phage Lysis Proteins and Their Targets. (Doctoral Dissertation)
- LaiHing, Steven 1983- (2011-08). Using High Throughput Screening to Acquire Promising Drug Candidates Against Mycobacterium tuberculosis. (Master's Thesis)