- Assistant Provost, Biochemistry and Biophysics, College of Agriculture and Life Sciences
We are currently investigating how organismal level cues regulate the onset of stem cell division during development. Our primary system is the neuroblasts in the brain of the fruit fly, Drosophila melanogaster. The trol gene of Drosophila encodes the fly homolog of the mammalian heparan sulfate glycoprotein, Perlecan. Perlecan is found in mice, humans, and C. elegans, and is widely known as a co-receptor for the growth factor FGF. We have shown that Trol, the Drosophila Perlecan homolog, is required for signaling by FGF. Furthermore, we have demonstrated that Trol is also a likely candidate for the Hedgehog co-receptor. Hedgehogs are peptide growth factors which are conserved in mammals and require heparan sulfate glycoproteins for their movement and long-range signaling; however, until now the identity of the protein core was unknown. Our studies demonstrate genetic interactions between trol and hedgehog or patched mutations (patched is the Hedgehog receptor). Further studies reveal that both FGF and Hedgehog signaling activate stem cell division. Current projects involve determining how Trol stimulates FGF and Hedgehog signaling through genetic, molecular, and biochemical analyses.
Academic Articles44
- Ferguson, B. W., & Datta, S. (2011). Role of heparan sulfate 2-o-sulfotransferase in prostate cancer cell proliferation, invasion, and growth factor signaling.. Prostate Cancer. 2011, 893208-14.
- Datta Kanjilal, S., & Datta, H. (2009). Subluxation of lens in Marfan syndrome.. Indian Pediatrics. 46(5), 434.
- Datta, S., Datta, H., & Kapoor, S. (2009). A case of psychogenic purpura in a female child.. Journal of the Indian Medical Association. 107(2), 104-106.
- Barrett, A. L., Krueger, S., & Datta, S. (2008). Branchless and Hedgehog operate in a positive feedback loop to regulate the initiation of neuroblast division in the Drosophila larval brain.. Developmental Biology. 317(1), 234-245.
- Barrett, A., Krueger, S., Jackson, M., & Datta, S. (2008). Curable genetic instability of a gal4 transgene in Drosophila.. Fly. 2(2), 67-73.
Conference Papers1
- DATTA, S (1995). CONTROL OF PROLIFERATION ACTIVATION IN QUIESCENT NEUROBLASTS OF THE DROSOPHILA CENTRAL-NERVOUS-SYSTEM. Journal of Cellular Biochemistry. 337-337.
- UGST311 Hnr-uscholar Exploration Serie Instructor
- UGST497 Capstone Instructor
- UGST497 Capstone-performance Instructor
- Lindner, Jonathan Ryan (2012-02). The Regulation of Growth Factor Signaling in Drosophila Development and Disease. (Doctoral Dissertation)
- Ferguson, Brent Wade (2012-02). Regulation of PERLECAN and 2OST Expression in Prostate Cancer Progression by Stress-activated Transcription Factors. (Doctoral Dissertation)
- Clarke, Douglas Nelson (2012-02). Perlecan Domain V Induces VEGF Secretion in Brain Endothelial Cells Through ?5?1 Integrin Dependent Mechanism a Novel Insight in Brain Tissue Recovery Following Ischemia. (Doctoral Dissertation)
- Barrett, Andrea Lynn (2009-05). A FGF-Hh feedback loop controls stem cell proliferation in the developing larval brain of drosophila melanogaster. (Doctoral Dissertation)
- Hernandez, Ana Maria (2009-05). Perlecan regulation of sonic hedgehog signaling: from drosophila to humans. (Doctoral Dissertation)