Analysis of phosphorylated isoforms of the p53 tumor suppressor protein in human lung carcinoma cells undergoing apoptosis | Academic Article individual record
abstract

The expression of p53 tumor suppressor protein isoforms in H460a cells induced to undergo apoptosis by 2-methoxyestradiol was investigated by two-dimensional gel electrophoresis. Whole-cell proteins from control and apoptotic H460a cells were separated by two-dimensional electrophoresis and were transferred to nitrocellulose. The p53 isoforms were detected by immunoblotting using p53 monoclonal antibody Bp53-12. Four isoforms of p53 (2, 3, 5, and 6) differing in phosphorylation state were detected in control cells. Three additional isoforms (1, 4, and 7) were observed to be expressed at significant levels only in apoptotic cells. The differential expression of isoforms 1, 4, and 7 in apoptotic cells suggests that one or more specific phosphorylation events generating these forms of p53 could play a role in regulating the function of p53 in apoptosis.

author list (cited authors)
Maxwell, S. A., Roth, J. A., & Mukhopadhyay, T.
publication date
1996
publisher
Wiley Publisher
published in
keywords
  • Tumor Cells, Cultured
  • Protein Processing, Post-Translational
  • Expression
  • Lung Cells
  • Electrophoresis, Gel, Two-Dimensional
  • Tumor Suppressor Protein P53
  • DNA Fragmentation
  • Phosphoproteins
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Neoplasm Proteins
  • Blotting, Western
  • Estradiol
  • Phosphorylation
  • Apoptosis
  • Lung Neoplasms
  • Carcinoma, Non-Small-Cell Lung
citation count

15