Immunogenicity of an Electron Beam Inactivated Rhodococcus equi Vaccine in Neonatal Foals | Academic Article individual record
abstract

Rhodococcus equi is an important pathogen of foals that causes severe pneumonia. To date, there is no licensed vaccine effective against R. equi pneumonia of foals. The objectives of our study were to develop an electron beam (eBeam) inactivated vaccine against R. equi and evaluate its immunogenicity. A dose of eBeam irradiation that inactivated replication of R. equi while maintaining outer cell wall integrity was identified. Enteral administration of eBeam inactivated R. equi increased interferon-γ production by peripheral blood mononuclear cells in response to stimulation with virulent R. equi and generated naso-pharyngeal R. equi-specific IgA in newborn foals. Our results indicate that eBeam irradiated R. equi administered enterally produce cell-mediated and upper respiratory mucosal immune responses, in the face of passively transferred maternal antibodies, similar to those produced in response to enteral administration of live organisms (a strategy which previously has been documented to protect foals against intrabronchial infection with virulent R. equi). No evidence of adverse effects was noted among vaccinated foals.

author list (cited authors)
Bordin, A. I., Pillai, S. D., Brake, C., Bagley, K. B., Bourquin, J. R., Coleman, M., ... Cohen, N. D.
publication date
2014
published in
PLoS ONE Journal
keywords
  • Immunity, Active
  • Bacterial Vaccines
  • Rhodococcus Equi
  • Actinomycetales Infections
  • Horses
  • Horse Diseases
  • Animals
altmetric score

1.0

citation count

5