Amino acid sequence of porcine heart fumarase | Academic Article individual record
abstract

The complete amino acid sequence of porcine heart fumarase (EC 4.2.1.2) has been determined from peptides produced by cyanogen bromide, endoproteinase Arg-C, S. aureus V8 protease, and trypsin. The enzyme is a tetramer of identical subunits with Mr = 50,015 and composed of 466 amino acid residues. Porcine heart fumarase displays 96% identity to human liver fumarase. Prediction of the secondary structural elements of porcine fumarase indicate that the enzyme contains a large amount of alpha helix with very little beta structure.

author list (cited authors)
Sacchettini, J. C., Frazier, M. W., Chiara, D. C., Banaszak, L. J., & Grant, G. A.
publication date
1988
publisher
Elsevier BV Publisher
published in
keywords
  • Fumarate Hydratase
  • Peptide Mapping
  • Myocardium
  • Molecular Sequence Data
  • Protein Conformation
  • Swine
  • Amino Acid Sequence
  • Animals
citation count

16