(500) Http failure response for https://api.library.tamu.edu/scholars-discovery/individual/search/findByIdIn?ids=n404152SE,n98150SE,n98049SE,n98062SE,n98133SE,n98171SE,n385680SE,n46132SE,n98204SE,n98109SE,n383092SE,n290721SE,n322241SE,n98126SE,n98149SE,n98132SE,n46121SE,n135261SE,n98155SE,n98084SE,n98027SE,n98061SE,n98056SE,n98188SE,n62956SE,n59741SE,n333080SE,n98161SE,n385861SE,n98203SE,n290688SE,n371176SE,n98022SE,n98095SE,n98089SE,n379805SE,n290672SE,n98194SE,n98121SE,n98177SE,n98078SE,n367445SE,n333079SE,n98110SE,n98166SE: 500 Internal Server Error
(404) Http failure response for https://api.library.tamu.edu/scholars-discovery/individual/n321765SE: 404 Not Found
Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing | Academic Article individual record

Several genes from prokaryotes and lower eukaryotes have been found to contain an in-frame open reading frame, which encodes for an internal protein (intein). Post-translationally, the internal polypeptide auto-splices and ligates the external sequences to yield a functional external protein (extein) and an intein. Most, but not all inteins, contain, apart from a splicing domain, a separate endonucleolytic domain that enables them to maintain their presence by a homing mechanism. We report here the crystal structure of an intein found in the gyrase A subunit from Mycobacterium xenopi at 2.2 A resolution. The structure contains an unusual beta-fold with the catalytic splice junctions at the ends of two adjacent antiparallel beta-strands. The arrangement of the active site residues Ser 1, Thr 72, His 75, His 197, and Asn 198 is consistent with a four-step mechanism for the cleavage-ligation reaction. Using site-directed mutagenesis, the N-terminal cysteine, proposed as the nucleophile in the first step of the splicing reaction, was changed to a Ser 1 and Ala 0, thus capturing the intein in a pre-spliced state.

author list (cited authors)
Klabunde, T., Sharma, S., Telenti, A., Jacobs, W. R., & Sacchettini, J. C.
publication date
  • Saccharomyces Cerevisiae Proteins
  • Insect Proteins
  • Dna Gyrase
  • Endonucleases
  • Mycobacterium Xenopi
  • Drosophila Proteins
  • Amino Acid Sequence
  • Dna Topoisomerases, Type Ii
  • Protein Splicing
  • Protein Structure, Tertiary
  • Deoxyribonucleases, Type II Site-Specific
  • Molecular Sequence Data
  • Protein Precursors
  • Saccharomyces Cerevisiae
  • Hedgehog Proteins
altmetric score


citation count