Crystal Structure of ATP Phosphoribosyltransferase fromMycobacterium tuberculosis | Academic Article individual record
abstract

The N-1-(5'-phosphoribosyl)-ATP transferase catalyzes the first step of the histidine biosynthetic pathway and is regulated by a feedback mechanism by the product histidine. The crystal structures of the N-1-(5'-phosphoribosyl)-ATP transferase from Mycobacterium tuberculosis in complex with inhibitor histidine and AMP has been determined to 1.8 A resolution and without ligands to 2.7 A resolution. The active enzyme exists primarily as a dimer, and the histidine-inhibited form is a hexamer. The structure represents a new fold for a phosphoribosyltransferase, consisting of three continuous domains. The inhibitor AMP binds in the active site cavity formed between the two catalytic domains. A model for the mechanism of allosteric inhibition has been derived from conformational differences between the AMP:His-bound and apo structures.

author list (cited authors)
Cho, Y., Sharma, V., & Sacchettini, J. C.
publication date
2003
keywords
  • Atp Phosphoribosyltransferase
  • Protein Structure, Quaternary
  • Mycobacterium Tuberculosis
  • Adenosine Monophosphate
  • Catalytic Domain
  • Cloning, Molecular
  • Models, Molecular
altmetric score

6.0

citation count

66