Reduced colitis-associated colon cancer in fat-1 (n-3 fatty acid desaturase) transgenic mice | Academic Article individual record

Bioactive food components containing n-3 polyunsaturated fatty acids (PUFA) modulate multiple determinants that link inflammation to cancer initiation and progression. Therefore, in this study, fat-1 transgenic mice,which convert endogenous n-6 PUFA to n-3 PUFA in multiple tissues,were injected with azoxymethane followed by three cycles of dextran sodium sulfate (DSS) to induce colitis-associated cancer. Fat-1 mice exhibited a reduced number of colonic adenocarcinomas per mouse (1.05 ± 0.29 vers us 2.12 ± 0.51, P = 0.033), elevated apoptosis (P = 0.03), and a decrease in n-6 PUFA-derived eicosanoids, compare d with wild-type (wt) mice. To determine whether the chemoprotective effects of n-3 PUFA could be attributed to its pleiotropic anti-inflammatory properties, colonic inflammation and injury scores were evaluated 5 days after DSS exposure followed by either a 3-day or 2-week recovery period. There was no effect of n-3 PUFA at 3 days. However,following a 2-week recovery period, colonic inflammation and ulceration scores returned to pretreatment levels compared with 3-day recovery only in fat-1 mice. For the purpose of examining the specific reactivity of lymphoid elements in the intestine,CD3 + T cells,CD4 + T helper cells, and macrophages from colonic lamina propria were quantified. Comparison of 3-day versus 2-week recovery time points revealed that fat-1 mice exhibited decreased (P < 0.05) CD3 + , CD4 + T helper, and macrophage cell numbers per colon as compared with wt mice. These results suggest that the antitumorigenic effect of n-3 PUFA may be mediated,in part, via its anti-inflammatory properties. ©2008 American Association for Cancer Research.

author list (cited authors)
Jia, Q., Lupton, J. R., Smith, R., Weeks, B. R., Callaway, E., Davidson, L. A., ... Chapkin, R. S.
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  • Colitis
  • Phospholipids
  • Antineoplastic Agents
  • Mice, Inbred C57BL
  • Colonic Neoplasms
  • Mice, Transgenic
  • Mice
  • Fatty Acids, Unsaturated
  • Lymphocytes
  • Antigens, CD4
  • Fatty Acid Desaturases
  • Apoptosis
  • CD4-Positive T-Lymphocytes
  • Antigens, CD3
  • Animals