Reduced colitis-associated colon cancer in fat-1 (n-3 fatty acid desaturase) transgenic mice | Academic Article individual record
abstract

Bioactive food components containing n-3 polyunsaturated fatty acids (PUFA) modulate multiple determinants that link inflammation to cancer initiation and progression. Therefore, in this study, fat-1 transgenic mice,which convert endogenous n-6 PUFA to n-3 PUFA in multiple tissues,were injected with azoxymethane followed by three cycles of dextran sodium sulfate (DSS) to induce colitis-associated cancer. Fat-1 mice exhibited a reduced number of colonic adenocarcinomas per mouse (1.05 ± 0.29 vers us 2.12 ± 0.51, P = 0.033), elevated apoptosis (P = 0.03), and a decrease in n-6 PUFA-derived eicosanoids, compare d with wild-type (wt) mice. To determine whether the chemoprotective effects of n-3 PUFA could be attributed to its pleiotropic anti-inflammatory properties, colonic inflammation and injury scores were evaluated 5 days after DSS exposure followed by either a 3-day or 2-week recovery period. There was no effect of n-3 PUFA at 3 days. However,following a 2-week recovery period, colonic inflammation and ulceration scores returned to pretreatment levels compared with 3-day recovery only in fat-1 mice. For the purpose of examining the specific reactivity of lymphoid elements in the intestine,CD3 + T cells,CD4 + T helper cells, and macrophages from colonic lamina propria were quantified. Comparison of 3-day versus 2-week recovery time points revealed that fat-1 mice exhibited decreased (P < 0.05) CD3 + , CD4 + T helper, and macrophage cell numbers per colon as compared with wt mice. These results suggest that the antitumorigenic effect of n-3 PUFA may be mediated,in part, via its anti-inflammatory properties. ©2008 American Association for Cancer Research.

author list (cited authors)
Jia, Q., Lupton, J. R., Smith, R., Weeks, B. R., Callaway, E., Davidson, L. A., ... Chapkin, R. S.
publication date
2008
published in
keywords
  • Colitis
  • Phospholipids
  • Antineoplastic Agents
  • Mice, Inbred C57BL
  • Colonic Neoplasms
  • Mice, Transgenic
  • Mice
  • Fatty Acids, Unsaturated
  • Lymphocytes
  • Antigens, CD4
  • Fatty Acid Desaturases
  • Apoptosis
  • CD4-Positive T-Lymphocytes
  • Antigens, CD3
  • Animals