Dynamics of Unfolded Nucleosomal Fiber | Academic Article individual record
abstract

The "rigidity" of chromatin fiber solenoidal structure in different states of condensation was evaluated with the help of gel-electrophoresis. A new property of the unfolded nucleosomal fiber-the capacity to condense with temperature-was demonstrated. These results together with our previously obtained data (W.A. Krajewski et al., Mol. Gen. Genet. 230, pp. 442-448, 1991; W.A. Krajewski et al., Ibid. 231, pp. 17-22, 1991) testify that changes in DNA linking number of transcriptionally active minichromosomes arise in vivo from alteration of nucleosomal solenoid parameters (i.e. from supernucleosomal level of chromatin organization), rather than from core histone modifications only or from increased flexibility of DNA within nucleosomes.

author list (cited authors)
Krajewski, W. A., Panin, V. M., & Razin, S. V.
publication date
1993
publisher
keywords
  • Nucleosomes
  • Electrophoresis, Polyacrylamide Gel
  • Histones
  • Rats
  • Chromatin
  • Cell Fractionation
  • DNA
  • Animals
  • Liver
  • Transcription, Genetic
  • Models, Structural
  • Nucleic Acid Conformation
citation count

5

PubMed ID
8357539
identifier
64778SE
Digital Object Identifier (DOI)
start page
1013
end page
1022
volume
10
issue
6