TRITHORAX-dependent arginine methylation of HSP68 mediates circadian repression by PERIOD in the monarch butterfly | Academic Article individual record
abstract

Significance Circadian repression drives the transcriptional feedback loops that keep circadian (∼24-h) time and synchronize an animal’s physiology and behavior to the daily environmental changes. Although PERIOD (PER) is known to initiate transcriptional repression by displacing the transcription activator CLOCK:BMAL1 from DNA, the underlying mechanism remains unknown. Using the monarch butterfly as a model harboring a simplified version of the mammalian circadian clock, we demonstrate that the binding of heat shock protein 68 (HSP68) to a region homologous to CLOCK mouse exon 19 is essential for CLK–PER interaction and PER repression. We further show that CLK–PER interaction and PER repression are promoted by the methylation of a single arginine methylation site (R45) on HSP68 via TRITHORAX catalytic activity.

authors
publication outlet

Proceedings of the National Academy of Sciences

author list (cited authors)
Zhang, Y., Iiams, S. E., Menet, J. S., Hardin, P. E., & Merlin, C
publication date
2022
keywords
  • Sleep Research
  • Generic Health Relevance
  • 1.1 Normal Biological Development And Functioning
  • Neurosciences
  • Genetics