Mammalian complex III heme dynamics studied with pump-probe spectroscopy and red light illuminations. | Academic Article individual record
abstract

The electronic or molecular mechanisms that initiate photobiomodulation (PBM) in cells are not yet fully understood. The porcine complex III (C-III) of the electron transport chain was characterized with transient absorption spectroscopy (TAS). We then applied our recently developed continuous wave laser coupled TAS procedure (CW-TAS) to investigate the effect of red light irradiances on the heme dynamics of C-III in its c1 reduced state. The time constants were found to be 3.30.3 ps for vibrational cooling of the oxidized state and 4.90.4 ps for rebinding of the photodissociated axial ligand of the c1 reduced state. The analysis of the CW-TAS procedure yielded no significant changes in the C-III heme dynamics. We rule out the possibility of 635 nm CW light at 4.7 mW/cm2 inducing a PBM effect on the heme dynamic of C-III, specifically with the photodissociation of its axial ligand.

publication outlet

Biomed Opt Express

author list (cited authors)
O'Connor, S. P., Powell, S. M., Rickman, J. M., Pope, N. J., Noojin, G. D., Scully, M. O., Denton, M. L., & Yakovlev, V. V.
publication date
2021
publisher
altmetric score

1.25

citation count

0

PubMed ID
34858701
identifier
585189SE
Digital Object Identifier (DOI)
start page
7082
end page
7091
volume
12
issue
11