Vaccination of yearling horses against poly-N-acetyl glucosamine fails to protect against infection with Streptococcus equi subspecies equi | Academic Article individual record
abstract

Strangles is a common disease of horses with worldwide distribution caused by the bacterium Streptococcus equi subspecies equi (SEE). Although vaccines against strangles are available commercially, these products have limitations in safety and efficacy. The microbial surface antigen β 1→6 poly-N-acetylglucosamine (PNAG) is expressed by SEE. Here we show that intramuscular (IM) injection alone or a combination of IM plus intranasal (IN) immunization generated antibodies to PNAG that functioned to deposit complement and mediate opsonophagocytic killing of SEE ex vivo. However, immunization strategies targeting PNAG either by either IM only injection or a combination of IM and IN immunizations failed to protect yearling horses against infection following contact with infected horses in an experimental setting. We speculate that a protective vaccine against strangles will require additional components, such as those targeting SEE enzymes that degrade or inactivate equine IgG.

author list (cited authors)
Cohen, N. D., Cywes-Bentley, C., Kahn, S. M., Bordin, A. I., Bray, J. M., Wehmeyer, S. G., & Pier, G. B.
publication date
2020
published in
PLoS One Journal
keywords
  • Acetylglucosamine
  • Streptococcus Equi
  • Animals
  • Horses
  • Streptococcal Infections
  • Male
  • Horse Diseases
  • Immunization
  • Vaccination
  • Injections, Intramuscular
  • Antibodies, Bacterial
  • Female
altmetric score

1.6

citation count

0

PubMed Central ID
33057397
identifier
495025SE
Digital Object Identifier (DOI)
start page
e0240479
end page
e0240479
volume
15
issue
10