Aspartate aminotransferase Rv3722c governs aspartate-dependent nitrogen metabolism in Mycobacterium tuberculosis | Academic Article individual record
abstract

Gene rv3722c of Mycobacterium tuberculosis is essential for in vitro growth, and encodes a putative pyridoxal phosphate-binding protein of unknown function. Here we use metabolomic, genetic and structural approaches to show that Rv3722c is the primary aspartate aminotransferase of M. tuberculosis, and mediates an essential but underrecognized role in metabolism: nitrogen distribution. Rv3722c deficiency leads to virulence attenuation in macrophages and mice. Our results identify aspartate biosynthesis and nitrogen distribution as potential species-selective drug targets in M. tuberculosis.

author list (cited authors)
Jansen, R. S., Mandyoli, L., Hughes, R., Wakabayashi, S., Pinkham, J. T., Selbach, B., ... Rhee, K. Y.
publication date
2020
published in
keywords
  • Basic Science
  • 2.1 Biological And Endogenous Factors
  • 0601 Biochemistry And Cell Biology
  • Orphan Drug
  • Infection
  • Infectious Diseases
  • Biodefense
  • Rare Diseases
  • Vaccine Related
  • Biomedical
  • Emerging Infectious Diseases
  • Prevention
  • 2.2 Factors Relating To Physical Environment
  • Tuberculosis