Mutations in the sigma subunit of E. coli RNA polymerase which affect positive control of transcription. | Academic Article individual record
abstract

The sigma subunits of bacterial RNA polymerases are required for the selective initiation of transcription. We have isolated and characterized mutations in rpoD, the gene which encodes the major form of sigma in E. coli, which affect the selectivity of transcription. These mutations increase the expression of araBAD up to 12-fold in the absence of CAP-cAMP. Expression of lac is unaffected, while expression of malT-activated operons is decreased. We determined the DNA sequence of 17 independently isolated mutations, and found that they consist of three different changes in a single CGC arginine codon at position 596 in the sigma polypeptide.

authors
author list (cited authors)
Hu, J. C., & Gross, C. A.
publication date
1985
published in
keywords
  • Arabinose
  • Mutation
  • Gene Expression Regulation
  • Escherichia Coli
  • Maltose
  • Sigma Factor
  • Transcription Factors
  • DNA-Directed RNA Polymerases
  • Receptors, Cyclic AMP
  • Base Sequence
  • Transcription, Genetic
  • Lac Operon
  • Operon
citation count

32