Marker rescue with plasmids bearing deletions in rpoD identifies a dispensable part of E. coli sigma factor. | Academic Article individual record
abstract

Recombinant DNA techniques allow rapid construction and characterization of tools for genetic dissection of essential genes. In this report, we describe construction of a set of deletions in rpoD, the gene which encodes the sigma subunit of E. coli RNA polymerase. We used this deletion set to identify a region of the sigma polypeptide which is dispensable for its function.

authors
author list (cited authors)
Hu, J. C., & Gross, C. A.
publication date
1983
published in
keywords
  • Transcription, Genetic
  • Escherichia Coli
  • Transcription Factors
  • Amino Acid Sequence
  • Chromosome Mapping
  • Sigma Factor
  • Structure-Activity Relationship
  • Chromosome Deletion
  • Genes
  • Genetic Engineering
  • Genes, Bacterial
  • Plasmids
citation count

31