| Academic Article individual record
abstract

Spermine (SPM) and its precursor putrescine (PUT), regulated by ornithine decarboxylase (ODC) and diamino-oxidase (DAO), are polyamines required for cell growth and proliferation. Only a few studies have investigated the anti-inflammatory and tumour inhibitory properties of probiotics on mucosal polyamine levels. We investigated the effects of a high concentration multistrain probiotic for human use on colonic polyamine biosynthesis in dogs. Histological sections (inflammatory bowel disease, n=10; polyposis, n=5) were assessed after receiving 112 to 225109 lyophilised bacteria daily for 60 days at baseline (T0) and 30 days after treatment end (T90). Histology scores, expression of PUT, SPM, ODC and DAO, and a clinical activity index (CIBDAI) were compared at T0 and T90. In polyps, cellular proliferation (Ki-67 expression), and apoptosis (caspase-3 protein expression) were also evaluated. After treatment, in inflammatory bowel disease significant decreases were observed for CIBDAI (P=0.006) and histology scores (P<0.001); PUT, SPM and ODC expression increased (P<0.01). In polyps, a significant decrease in polyamine levels, ODC activity, and Ki-67, and a significant increase in caspase-3 positivity and DAO expression (P=0.005) was noted. Our results suggest potential anti-proliferative and anti-inflammatory effects of the probiotic mixture in polyps and inflammation, associated with reduced mucosal infiltration and up-regulation of PUT, SPM, and ODC levels.

publication outlet

Benef Microbes

author list (cited authors)
Rossi, G., Cerquetella, M., Scarpona, S., Pengo, G., Fettucciari, K., Bassotti, G., Jergens, A. E., & Suchodolski, J. S.
publication date
2018
keywords
  • Animals
  • Colonic Polyps
  • Caspase 3
  • Inflammatory Bowel Diseases
  • Putrescine
  • Polyps
  • Spermine
  • Ornithine Decarboxylase
  • Bacterial Physiological Phenomena
  • Probiotic
  • Dog Diseases
  • Treatment Outcome
  • Ki-67 Antigen
  • Probiotics
  • Colon
  • Polyamines
  • Mucosal Histology
  • Dogs
  • Amine Oxidase (copper-containing)
  • Gene Expression Regulation
Digital Object Identifier (DOI)
issue
2