ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR CANINE SERUM FERRITIN, USING MONOCLONAL ANTI-CANINE FERRITIN IMMUNOGLOBULIN-G | Academic Article individual record
abstract

Immunoassay of serum ferritin is currently used to evaluate the clinical iron status of human beings, horses, cattle, and swine. Because ferritins are immunologically species specific, a separate assay must be developed for each species. We have developed an ELISA for serum ferritin in dogs, using a monoclonal anti-canine ferritin antibody. Ferritin standards were linear (r = 0.997) from 0 to 80 ng/ml. Recovery of ferritin from canine serum was 94%. Dilutions of pooled canine serum were linear from 0 to 50% (r = 0.994). Within-assay coefficient of variability was 5.5%, whereas assay-to-assay coefficient of variability ranged from 12.5 to 21%. This assay should provide a nonsurgical means of accurately estimating dogs' iron stores.

author list (cited authors)
WEEKS, B. R., SMITH, J. E., & PHILLIPS, R. M.
publication date
1988
keywords
  • Species Specificity
  • Ferritins
  • Antibodies, Monoclonal
  • Enzyme-Linked Immunosorbent Assay
  • Dogs
  • Immunoglobulin G
  • Animals
citation count

12