Determination of acetaldehyde in blood by solid phase extraction and high performance liquid chromatography | Academic Article individual record
abstract

A simple method has been developed for the measurement of acetaldehyde in blood. Samples were treated with perchloric acid to precipitate proteins. After centrifugation, the supernatant, together with the internal standard (crotonaldehyde), were reacted with 2,4-dinitrophenylhydrazine reagent. The derivatized acetaldehyde was then isolated by solid phase extraction and followed by high performance liquid chromatography quantitation. This method had a minimal detectable concentration of 0.28 microM, and displayed an intra-assay precision of 2.23% and an inter-assay precision of 5.18%. The recoveries of acetaldehyde at added concentrations of 1.42 and 7.14 microM were 106.7% and 101.9%, respectively.

author list (cited authors)
Ma, W. D., & Klemm, W. R.
publication date
1997
publisher
Elsevier bv Publisher
published in
ALCOHOL Journal
keywords
  • High Performance Liquid Chromatography
  • Blood
  • Solid Phase Extraction
  • ACETALDEHYDE
citation count

10