Production of human monoclonal antibody in eggs of chimeric chickens | Academic Article individual record
abstract

The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and downstream DNA sequences of ovalbumin, a protein produced exclusively in very high quantities in chicken egg white, to drive tissue-specific expression of human mAb in chicken eggs. To accommodate these large regulatory regions, we established and transfected lines of chicken embryonic stem (cES) cells and formed chimeras that express mAb from cES cell-derived tubular gland cells. Eggs from high-grade chimeras contained up to 3 mg of mAb that possesses enhanced antibody-dependent cellular cytotoxicity (ADCC), nonantigenic glycosylation, acceptable half-life, excellent antigen recognition and good rates of internalization.

author list (cited authors)
Zhu, L., van de Lavoir, M., Albanese, J., Beenhouwer, D. O., Cardarelli, P. M., Cuison, S., ... Etches, R. J.
publication date
2005
published in
keywords
  • Glycosylation
  • Polymerase Chain Reaction
  • Calorimetry, Differential Scanning
  • Antibodies, Monoclonal
  • Oligosaccharides
  • Genome
  • Spectrometry, Mass, Electrospray Ionization
  • Animals
  • Immunohistochemistry
  • Embryo, Nonmammalian
  • Chickens
  • Monosaccharides
  • Stem Cells
  • Models, Genetic
  • Embryo, Mammalian
  • Blotting, Western
  • Cricetinae
  • Reverse Transcriptase Polymerase Chain Reaction
  • Immunoglobulin G
  • CHO Cells
  • Enzyme-Linked Immunosorbent Assay
  • Blotting, Southern
  • Humans
  • Mice
  • Ovalbumin
  • Recombinant Fusion Proteins
  • Genetic Vectors
  • DNA
  • Female
  • Mice, Inbred BALB C
  • Egg White
  • Carbohydrates
  • Isoelectric Focusing
altmetric score

14.008

citation count

159