Brillouin spectroscopy reveals changes in muscular viscoelasticity in Drosophila POMT mutants | Conference Paper individual record
abstract

© 2015 SPIE. Muscular dystrophy (MD) is a group of muscle diseases that induce weakness in skeletal muscle and cause progressive muscle degeneration. The muscular mechanical properties (i.e., viscoelasticity), however, have not been thoroughly examined before and after MD. On the other hand, Brillouin spectroscopy (BS) provides a non-invasive approach to probing the local sound speed within a small volume. Moreover, recent advances in background-free Brillouin spectroscopy enable investigators to imaging not only transparent samples, but also turbid ones. In this study, we investigated the mechanical properties of muscles while employing Drosophila model of dystroglycanopathies, human congenital muscular dystrophies resulting from abnormal glycosylation of alpha-dystroglycan. Specifically, we analyzed larval abdominal muscles of Drosophila with mutations in protein O-mannosyltransferase (POMT) genes. As a comparison, we have also examined muscular tissues dissected from wildtype Drosophila. The Brillouin spectra were obtained by a background free VIPA (virtually imaged phased array) spectrometer described in the previous report. As a reference, the Raman spectra were also acquired for each test. Our current results indicated that POMT defects cause changes in muscle elasticity, which suggests that muscular dystrophy conditions may be also associated with abnormalities in muscle elastic properties.

author list (cited authors)
Meng, Z., Baker, R., Panin, V. M., & Yakovlev, V. V.
publication date
2015
publisher
SPIE Publisher
keywords
  • Muscular Elasticity
  • Muscular Dystrophy
  • Brillouin Spectroscopy
  • Drosophila
citation count

4