A cell-counting factor regulating structure size in Dictyostelium. | Academic Article individual record
abstract

Developing Dictyostelium cells form large aggregation streams that break up into groups of 0.2 x 10(5) to 1 x 10(5) cells. Each group then becomes a fruiting body. smlA cells oversecrete an unknown factor that causes aggregation streams to break up into groups of approximately 5 x 10(3) cells and thus form very small fruiting bodies. We have purified the counting factor and find that it behaves as a complex of polypeptides with an effective molecular mass of 450 kD. One of the polypeptides is a 40-kD hydrophilic protein we have named counting. In transformants with a disrupted counting gene, there is no detectable secretion of counting factor, and the aggregation streams do not break up, resulting in huge (up to 2 x 10(5) cell) fruiting bodies.

author list (cited authors)
Brock, D. A., & Gomer, R. H.
publication date
1999
published in
keywords
  • Dictyostelium
  • Cloning, Molecular
  • Animals
  • Protozoan Proteins
  • Mutation
  • Signal Transduction
  • Cell Aggregation
  • Molecular Weight
  • Base Sequence
  • Culture Media, Conditioned
  • Sequence Analysis
  • Phenotype
  • RNA, Messenger
  • Protein Sorting Signals
  • Molecular Sequence Data
  • Cell Count
  • Amino Acid Sequence
citation count

97