U-3'-BCIP - A CHROMOGENIC SUBSTRATE FOR THE DETECTION OF RNASE-A IN RECOMBINANT-DNA EXPRESSION SYSTEMS | Academic Article individual record
abstract

The synthesis of the bovine pancreatic ribonuclease A (RNase A, EC 3.1.27.5) chromogenic substrate uridine-3'-(5-bromo-4-chloroindol-3-yl)-phosphate (U-3'-BCIP) is described. RNase A catalyzes the hydrolysis of U-3'-BCIP to release a halogenated indol-3-ol that undergoes rapid aerobic oxidation to the dark blue 5,5'-dibromo-4,4'-dichloroindigo. Preliminary kinetic studies indicate that this compound may have practical use for assaying RNase A activity both in vitro and in vivo, e.g. in screening bacterial colonies for RNase A produced by recombinant DNA methods.

authors
author list (cited authors)
WITMER, M. R., FALCOMER, C. M., WEINER, M. P., KAY, M. S., BEGLEY, T. P., GANEM, B., & SCHERAGA, H. A.
publication date
1991
published in
keywords
  • Uridine Monophosphate
  • Ribonuclease, Pancreatic
  • DNA, Recombinant
  • Oxidation-Reduction
  • Molecular Structure
  • Substrate Specificity
  • Cattle
  • Animals
  • Kinetics
altmetric score

6.0

citation count

19