Culture of Tumorigenic Cells on Protein Fibers Reveals Metastatic Cell Behaviors | Academic Article individual record
abstract

Tumorigenic cell behaviors can be suppressed or enhanced by their physicochemical environment. As a first step toward developing materials that allow tumorigenic behaviors to be observed and manipulated, we cultured related MCF10 breast cell lines on fibers composed of the Drosophila protein Ultrabithorax (Ubx). These cell lines, originally derived from fibrocystic breast tissue, represent a continuum of tumorigenic behavior. Immortal but nontumorigenic MCF10A cells, as well as semitumorigenic MCF10AT cells, attached and spread on Ubx fibers. MCF10CA-1a cells, the most highly transformed line, secreted high concentrations of matrix metalloproteinases when cultured on Ubx materials, resulting in differences in cell attachment and cytoskeletal structure, and enabling invasive behavior. Because the mechanical and functional properties of Ubx fibers can be genetically manipulated, these materials provide a valuable tool for cancer research, allowing creation of diverse microenvironments that allow assessment of invasive, metastatic behavior.

author list (cited authors)
Hsiao, H., Santos, A., Howell, D. W., Patterson, J. L., Fuchs-Young, R., & Bondos, S. E.
publication date
2016
published in
keywords
  • Neoplasm Metastasis
  • Homeodomain Proteins
  • Transcription Factors
  • Breast Neoplasms
  • Drosophila Melanogaster
  • Female
  • Cell Line, Tumor
  • Animals
  • Drosophila Proteins
  • Cell Culture Techniques
  • Humans
altmetric score

0.5

citation count

3