RASA contains a polymorphic microsatellite and maps to bovine syntenic group U22 on chromosome 7q2.4-qter.
|
Academic Article
individual record
abstract
The bovine gene for the p21ras protein activator (RASA) includes in its 5' untranslated region a (TG)n repeat. Analysis of this (TG)n repeat by PCR amplification of genomic DNA revealed a four-allele polymorphism. A cDNA probe was used to assign RASA to the region 2.4-qter of bovine Chromosome (Chr) 7 by in situ hybridization. PCR analysis of a panel of somatic hybrid lines allowed the assignment of RASA to the unassigned syntenic group 22 (U22) and thus localizes U22 on Chr 7.
authors
publication outlet
Mamm Genome
author list (cited authors)
Eggen, A., Solinas-Toldo, S., Dietz, A. B., Womack, J. E., Stranzinger, G., & Fries, R.
publication date
1992
publisher
Springer Nature
Publisher
keywords
- In Situ Hybridization
- Chromosome Mapping
- DNA, Satellite
- Proteins
- CHO Cells
- Polymorphism, Genetic
- Cricetinae
- Base Sequence
- Molecular Sequence Data
- Hybrid Cells
- Cattle
- Animals
- Male
- Proto-Oncogene Proteins P21(ras)
- Chromosome Banding
citation count
12
identifier
75081SE
Digital Object Identifier (DOI)
start page
559
end page
563
volume
3
issue
10